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Transmission studies of the newly described apple chlorotic fruit spot viroid using a combined RT-qPCR and droplet digital PCR approach

The transmission of the apscaviroid tentatively named apple chlorotic fruit spot viroid (ACFSVd) was investigated using a one-step reverse-transcription (RT) droplet digital PCR assay for absolute quantification of the viroid, followed by quantification of relative standard curves by RT-qPCR. Our re...

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Detalles Bibliográficos
Autores principales: Leichtfried, Thomas, Reisenzein, Helga, Steinkellner, Siegrid, Gottsberger, Richard A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7547948/
https://www.ncbi.nlm.nih.gov/pubmed/32638117
http://dx.doi.org/10.1007/s00705-020-04704-5