Transmission studies of the newly described apple chlorotic fruit spot viroid using a combined RT-qPCR and droplet digital PCR approach

The transmission of the apscaviroid tentatively named apple chlorotic fruit spot viroid (ACFSVd) was investigated using a one-step reverse-transcription (RT) droplet digital PCR assay for absolute quantification of the viroid, followed by quantification of relative standard curves by RT-qPCR. Our re...

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Autores principales: Leichtfried, Thomas, Reisenzein, Helga, Steinkellner, Siegrid, Gottsberger, Richard A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2020
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7547948/
https://www.ncbi.nlm.nih.gov/pubmed/32638117
http://dx.doi.org/10.1007/s00705-020-04704-5
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author Leichtfried, Thomas
Reisenzein, Helga
Steinkellner, Siegrid
Gottsberger, Richard A.
author_facet Leichtfried, Thomas
Reisenzein, Helga
Steinkellner, Siegrid
Gottsberger, Richard A.
author_sort Leichtfried, Thomas
collection PubMed
description The transmission of the apscaviroid tentatively named apple chlorotic fruit spot viroid (ACFSVd) was investigated using a one-step reverse-transcription (RT) droplet digital PCR assay for absolute quantification of the viroid, followed by quantification of relative standard curves by RT-qPCR. Our results indicate that ACFSVd is effectively transmitted by grafting, budding and seeds. No transmission has yet been observed to the viroid-inoculated pome fruit species Pyrus sp. and Cydonia sp. ACFSVd was detected in viruliferous aphids (Myzus persicae, Dysaphis plantaginea) and in codling moths (Cydia pomonella). The viroid was also detected systemically in the infected hemiparasitic plant Viscum album subsp. album (mistletoe). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00705-020-04704-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-75479482020-10-19 Transmission studies of the newly described apple chlorotic fruit spot viroid using a combined RT-qPCR and droplet digital PCR approach Leichtfried, Thomas Reisenzein, Helga Steinkellner, Siegrid Gottsberger, Richard A. Arch Virol Brief Report The transmission of the apscaviroid tentatively named apple chlorotic fruit spot viroid (ACFSVd) was investigated using a one-step reverse-transcription (RT) droplet digital PCR assay for absolute quantification of the viroid, followed by quantification of relative standard curves by RT-qPCR. Our results indicate that ACFSVd is effectively transmitted by grafting, budding and seeds. No transmission has yet been observed to the viroid-inoculated pome fruit species Pyrus sp. and Cydonia sp. ACFSVd was detected in viruliferous aphids (Myzus persicae, Dysaphis plantaginea) and in codling moths (Cydia pomonella). The viroid was also detected systemically in the infected hemiparasitic plant Viscum album subsp. album (mistletoe). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00705-020-04704-5) contains supplementary material, which is available to authorized users. Springer Vienna 2020-07-08 2020 /pmc/articles/PMC7547948/ /pubmed/32638117 http://dx.doi.org/10.1007/s00705-020-04704-5 Text en © The Author(s) 2020, corrected publication 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Brief Report
Leichtfried, Thomas
Reisenzein, Helga
Steinkellner, Siegrid
Gottsberger, Richard A.
Transmission studies of the newly described apple chlorotic fruit spot viroid using a combined RT-qPCR and droplet digital PCR approach
title Transmission studies of the newly described apple chlorotic fruit spot viroid using a combined RT-qPCR and droplet digital PCR approach
title_full Transmission studies of the newly described apple chlorotic fruit spot viroid using a combined RT-qPCR and droplet digital PCR approach
title_fullStr Transmission studies of the newly described apple chlorotic fruit spot viroid using a combined RT-qPCR and droplet digital PCR approach
title_full_unstemmed Transmission studies of the newly described apple chlorotic fruit spot viroid using a combined RT-qPCR and droplet digital PCR approach
title_short Transmission studies of the newly described apple chlorotic fruit spot viroid using a combined RT-qPCR and droplet digital PCR approach
title_sort transmission studies of the newly described apple chlorotic fruit spot viroid using a combined rt-qpcr and droplet digital pcr approach
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7547948/
https://www.ncbi.nlm.nih.gov/pubmed/32638117
http://dx.doi.org/10.1007/s00705-020-04704-5
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