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Transmission studies of the newly described apple chlorotic fruit spot viroid using a combined RT-qPCR and droplet digital PCR approach
The transmission of the apscaviroid tentatively named apple chlorotic fruit spot viroid (ACFSVd) was investigated using a one-step reverse-transcription (RT) droplet digital PCR assay for absolute quantification of the viroid, followed by quantification of relative standard curves by RT-qPCR. Our re...
Autores principales: | Leichtfried, Thomas, Reisenzein, Helga, Steinkellner, Siegrid, Gottsberger, Richard A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Vienna
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7547948/ https://www.ncbi.nlm.nih.gov/pubmed/32638117 http://dx.doi.org/10.1007/s00705-020-04704-5 |
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