Cargando…

Optimization of Methods for the Production and Refolding of Biologically Active Disulfide Bond-Rich Antibody Fragments in Microbial Hosts

Antibodies have been used for basic research, clinical diagnostics, and therapeutic applications. Escherichia coli is one of the organisms of choice for the production of recombinant antibodies. Variable antibody genes have canonical and non-canonical disulfide bonds that are formed by the oxidation...

Descripción completa

Detalles Bibliográficos
Autores principales: Ban, Bhupal, Sharma, Maya, Shetty, Jagathpala
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551518/
https://www.ncbi.nlm.nih.gov/pubmed/32764309
http://dx.doi.org/10.3390/antib9030039