Cargando…

Capturing Membrane Protein Ribosome Nascent Chain Complexes in a Native-like Environment for Co-translational Studies

[Image: see text] Co-translational folding studies of membrane proteins lag behind cytosolic protein investigations largely due to the technical difficulty in maintaining membrane lipid environments for correct protein folding. Stalled ribosome-bound nascent chain complexes (RNCs) can give snapshots...

Descripción completa

Detalles Bibliográficos
Autores principales:	Pellowe, Grant A., Findlay, Heather E., Lee, Karen, Gemeinhardt, Tim M., Blackholly, Laura R., Reading, Eamonn, Booth, Paula J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2020
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551657/ https://www.ncbi.nlm.nih.gov/pubmed/32627541 http://dx.doi.org/10.1021/acs.biochem.0c00423

Ejemplares similares

Nuclear translation visualized by ribosome-bound nascent chain puromycylation
por: David, Alexandre, et al.
Publicado: (2012)

Co-translational capturing of nascent ribosomal proteins by their dedicated chaperones
por: Pausch, Patrick, et al.
Publicado: (2015)

Methods to study folding of alpha-helical membrane proteins in lipids
por: Harris, Nicola J., et al.
Publicado: (2022)

Nascent SecM Chain Outside the Ribosome Reinforces Translation Arrest
por: Yang, Zhuohao, et al.
Publicado: (2015)

Selective stalling of human translation through small-molecule engagement of the ribosome nascent chain
por: Lintner, Nathanael G., et al.
Publicado: (2017)

Nascent chain dynamics and ribosome interactions within folded ribosome–nascent chain complexes observed by NMR spectroscopy
por: Burridge, Charles, et al.
Publicado: (2021)

Cell-Free Expression to Probe Co-Translational Insertion of an Alpha Helical Membrane Protein
por: Blackholly, Laura R., et al.
Publicado: (2022)

Correction: Selective stalling of human translation through small-molecule engagement of the ribosome nascent chain
por: Lintner, Nathanael G., et al.
Publicado: (2018)

Immunoprecipitation of Reporter Nascent Chains from Active Ribosomes to Study Translation Efficiency
por: Cacioppo, Roberta, et al.
Publicado: (2023)

Nascent SecM chain interacts with outer ribosomal surface to stabilize translation arrest
por: Muta, Mikihisa, et al.
Publicado: (2020)

Ribosomes have a NAC for nascent chain processing
por: Short, Ben
Publicado: (2015)

NAC covers ribosome-associated nascent chains thereby forming a protective environment for regions of nascent chains just emerging from the peptidyl transferase center
Publicado: (1995)

MifM-instructed translation arrest involves nascent chain interactions with the exterior as well as the interior of the ribosome
por: Fujiwara, Keigo, et al.
Publicado: (2018)

A structural ensemble of a ribosome-nascent chain complex during co-translational protein folding
por: Cabrita, Lisa D., et al.
Publicado: (2016)

MITRAC15/COA1 promotes mitochondrial translation in a ND2 ribosome–nascent chain complex
por: Wang, Cong, et al.
Publicado: (2019)

Cell-free expression tools to study co-translational folding of alpha helical membrane transporters
por: Harris, Nicola J., et al.
Publicado: (2020)

Common sequence motifs of nascent chains engage the ribosome surface and trigger factor
por: Deckert, Annika, et al.
Publicado: (2021)

Hydrogen-deuterium exchange mass spectrometry captures distinct dynamics upon substrate and inhibitor binding to a transporter
por: Jia, Ruyu, et al.
Publicado: (2020)

Interactions between nascent proteins and the ribosome surface inhibit co-translational folding
por: Cassaignau, Anaïs M. E., et al.
Publicado: (2021)

Nascent mutant Huntingtin exon 1 chains do not stall on ribosomes during translation but aggregates do recruit machinery involved in ribosome quality control and RNA
por: Ormsby, Angelique R., et al.
Publicado: (2020)

Nascent polypeptide chains exit the ribosome in the same relative position in both eucaryotes and procaryotes
Publicado: (1983)

Identification of a Novel Stage of Ribosome/Nascent Chain Association with the Endoplasmic Reticulum Membrane
por: Murphy, Edwin C., et al.
Publicado: (1997)

Elongation inhibitors do not prevent the release of puromycylated nascent polypeptide chains from ribosomes
por: Hobson, Benjamin D, et al.
Publicado: (2020)

The ribosome-associated complex RAC serves in a relay that directs nascent chains to Ssb
por: Zhang, Ying, et al.
Publicado: (2020)

A Possible Role of the Full-Length Nascent Protein in Post-Translational Ribosome Recycling
por: Das, Debasis, et al.
Publicado: (2017)

Shaping the Nascent Ribosome: AAA-ATPases in Eukaryotic Ribosome Biogenesis
por: Prattes, Michael, et al.
Publicado: (2019)

Membrane-bound ribosomes of myeloma cells. III. The role of the messenger RNA and the nascent polypeptide chain in the binding of ribosomes to membranes
Publicado: (1975)

Multivalent contacts of the Hsp70 Ssb contribute to its architecture on ribosomes and nascent chain interaction
por: Hanebuth, Marie A., et al.
Publicado: (2016)

How lipids affect the energetics of co-translational alpha helical membrane protein folding
por: Brady, Ryan, et al.
Publicado: (2022)

S-Adenosyl-l-methionine Induces Compaction of Nascent Peptide Chain inside the Ribosomal Exit Tunnel upon Translation Arrest in the Arabidopsis CGS1 Gene
por: Onoue, Noriyuki, et al.
Publicado: (2011)

Structural basis for selective stalling of human ribosome nascent chain complexes by a drug-like molecule
por: Li, Wenfei, et al.
Publicado: (2019)

Coordination of -1 programmed ribosomal frameshifting by transcript and nascent chain features revealed by deep mutational scanning
por: Carmody, Patrick J, et al.
Publicado: (2021)

Ribosome selectivity and nascent chain context in modulating the incorporation of fluorescent non-canonical amino acid into proteins
por: Thommen, Michael, et al.
Publicado: (2022)

The Rqc2/Tae2 subunit of the ribosome-associated quality control (RQC) complex marks ribosome-stalled nascent polypeptide chains for aggregation
por: Yonashiro, Ryo, et al.
Publicado: (2016)

Defining the Specificity of Cotranslationally Acting Chaperones by Systematic Analysis of mRNAs Associated with Ribosome-Nascent Chain Complexes
por: del Alamo, Marta, et al.
Publicado: (2011)

Increasing the sensitivity of NMR diffusion measurements by paramagnetic longitudinal relaxation enhancement, with application to ribosome–nascent chain complexes
por: Chan, Sammy H. S., et al.
Publicado: (2015)

Vms1/Ankzf1 peptidyl-tRNA hydrolase releases nascent chains from stalled ribosomes
por: Verma, Rati, et al.
Publicado: (2018)

Critical 23S rRNA interactions for macrolide-dependent ribosome stalling on the ErmCL nascent peptide chain
por: Koch, Miriam, et al.
Publicado: (2017)

The dynamic architecture of Map1- and NatB-ribosome complexes coordinates the sequential modifications of nascent polypeptide chains
por: Knorr, Alexandra G., et al.
Publicado: (2023)

The Ribosome Restrains Molten Globule Formation in Stalled Nascent Flavodoxin
por: Houwman, Joseline A., et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_88e48lgs5f135uq5id3f4qh58v