Quantitative Microdialysis: Experimental Protocol and Software for Small Molecule Protein Affinity Determination and for Exclusion of Compounds with Poor Physicochemical Properties

Quantitative microdialysis is a traditional biophysical affinity determination technique. In the development of the detailed experimental protocol presented, we used commercially available equipment, rapid equilibrium dialysis (RED) devices (ThermoFisher Scientific), which means that it is open to m...

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Detalles Bibliográficos
Autores principales: Shave, Steven, Pham, Nhan T., Śmieja, Connor B., Auer, Manfred
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563421/
https://www.ncbi.nlm.nih.gov/pubmed/32751503
http://dx.doi.org/10.3390/mps3030055

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563421/
https://www.ncbi.nlm.nih.gov/pubmed/32751503
http://dx.doi.org/10.3390/mps3030055

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