Cargando…
Recent trends in the utilization of LAMP for the diagnosis of viruses, bacteria, and allergens in food
Foodborne diseases are becoming major scientific struggles for both developing and developed countries. Most foodborne infections are caused by microbial pathogens such as bacteria, viruses, and other food allergens. Hence early and accurate diagnosis of these foodborne pathogens is always preferabl...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564122/ http://dx.doi.org/10.1016/B978-0-12-821406-0.00027-8 |
_version_ | 1783595641681739776 |
---|---|
author | Lakshmi, Buddolla Anantha Kim, Sanghyo |
author_facet | Lakshmi, Buddolla Anantha Kim, Sanghyo |
author_sort | Lakshmi, Buddolla Anantha |
collection | PubMed |
description | Foodborne diseases are becoming major scientific struggles for both developing and developed countries. Most foodborne infections are caused by microbial pathogens such as bacteria, viruses, and other food allergens. Hence early and accurate diagnosis of these foodborne pathogens is always preferable. To satisfy these concerns, plenty of isothermal amplification methodologies such as rolling circle amplification (RCA), helicase-dependent amplification (HDA), and recombinase polymerase amplification (RPA) have been developed. Among these, loop-mediated isothermal amplification (LAMP) is a widely usable, potential, and recognizable amplification technique achieved at a constant temperature around 60°C within 30–60 min by using only one kind of enzyme. As a robust gene amplification technique, it can be employed for the detection of bacteria, viruses, and other related food allergens. This technique has its own merits such as cost-effectiveness, facile manufacturing procedure, and consistency. In this chapter, we emphasize recent trends in designing the techniques, challenges, and the future prospects of LAMP in the detection of foodborne pathogens. These effective pathogen detection methods may offer potential benefits compared with existing conventional methods. |
format | Online Article Text |
id | pubmed-7564122 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
record_format | MEDLINE/PubMed |
spelling | pubmed-75641222020-10-16 Recent trends in the utilization of LAMP for the diagnosis of viruses, bacteria, and allergens in food Lakshmi, Buddolla Anantha Kim, Sanghyo Recent Developments in Applied Microbiology and Biochemistry Article Foodborne diseases are becoming major scientific struggles for both developing and developed countries. Most foodborne infections are caused by microbial pathogens such as bacteria, viruses, and other food allergens. Hence early and accurate diagnosis of these foodborne pathogens is always preferable. To satisfy these concerns, plenty of isothermal amplification methodologies such as rolling circle amplification (RCA), helicase-dependent amplification (HDA), and recombinase polymerase amplification (RPA) have been developed. Among these, loop-mediated isothermal amplification (LAMP) is a widely usable, potential, and recognizable amplification technique achieved at a constant temperature around 60°C within 30–60 min by using only one kind of enzyme. As a robust gene amplification technique, it can be employed for the detection of bacteria, viruses, and other related food allergens. This technique has its own merits such as cost-effectiveness, facile manufacturing procedure, and consistency. In this chapter, we emphasize recent trends in designing the techniques, challenges, and the future prospects of LAMP in the detection of foodborne pathogens. These effective pathogen detection methods may offer potential benefits compared with existing conventional methods. 2021 2020-10-16 /pmc/articles/PMC7564122/ http://dx.doi.org/10.1016/B978-0-12-821406-0.00027-8 Text en Copyright © 2021 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Lakshmi, Buddolla Anantha Kim, Sanghyo Recent trends in the utilization of LAMP for the diagnosis of viruses, bacteria, and allergens in food |
title | Recent trends in the utilization of LAMP for the diagnosis of viruses, bacteria, and allergens in food |
title_full | Recent trends in the utilization of LAMP for the diagnosis of viruses, bacteria, and allergens in food |
title_fullStr | Recent trends in the utilization of LAMP for the diagnosis of viruses, bacteria, and allergens in food |
title_full_unstemmed | Recent trends in the utilization of LAMP for the diagnosis of viruses, bacteria, and allergens in food |
title_short | Recent trends in the utilization of LAMP for the diagnosis of viruses, bacteria, and allergens in food |
title_sort | recent trends in the utilization of lamp for the diagnosis of viruses, bacteria, and allergens in food |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564122/ http://dx.doi.org/10.1016/B978-0-12-821406-0.00027-8 |
work_keys_str_mv | AT lakshmibuddollaanantha recenttrendsintheutilizationoflampforthediagnosisofvirusesbacteriaandallergensinfood AT kimsanghyo recenttrendsintheutilizationoflampforthediagnosisofvirusesbacteriaandallergensinfood |