Using High-Content Screening to Generate Single-Cell Gene-Corrected Patient-Derived iPS Clones Reveals Excess Alpha-Synuclein with Familial Parkinson’s Disease Point Mutation A30P

The generation of isogenic induced pluripotent stem cell (iPSC) lines using CRISPR-Cas9 technology is a technically challenging, time-consuming process with variable efficiency. Here we use fluorescence-activated cell sorting (FACS) to sort biallelic CRISPR-Cas9 edited single-cell iPSC clones into h...

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Detalles Bibliográficos
Autores principales: Barbuti, Peter, Antony, Paul, Santos, Bruno, Massart, François, Cruciani, Gérald, Dording, Claire, Arias, Jonathan, Schwamborn, Jens, Krüger, Rejko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564375/
https://www.ncbi.nlm.nih.gov/pubmed/32927687
http://dx.doi.org/10.3390/cells9092065

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564375/
https://www.ncbi.nlm.nih.gov/pubmed/32927687
http://dx.doi.org/10.3390/cells9092065

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