Dynamic High-Sensitivity Quantitation of Procollagen-I by Endogenous CRISPR-Cas9 NanoLuciferase Tagging

The ability to quantitate a protein of interest temporally and spatially at subcellular resolution in living cells would generate new opportunities for research and drug discovery, but remains a major technical challenge. Here, we describe dynamic, high-sensitivity protein quantitation technique usi...

Descripción completa

Detalles Bibliográficos
Autores principales: Calverley, Ben C., Kadler, Karl E., Pickard, Adam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564849/
https://www.ncbi.nlm.nih.gov/pubmed/32927811
http://dx.doi.org/10.3390/cells9092070

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564849/
https://www.ncbi.nlm.nih.gov/pubmed/32927811
http://dx.doi.org/10.3390/cells9092070

Ejemplares similares