Genetically encoded protein sulfation in mammalian cells

Tyrosine sulfation is an important post-translational modification found in higher eukaryotes. Here we report an engineered tyrosyl-tRNA synthetase/tRNA pair that co-translationally incorporates O-sulfotyrosine in response to UAG codons in E. coli and mammalian cells. This platform enables recombina...

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Autores principales: Italia, James S., Peeler, Jennifer C., Hillenbrand, Christen M., Latour, Christopher, Weerapana, Eranthie, Chatterjee, Abhishek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564891/
https://www.ncbi.nlm.nih.gov/pubmed/32198493
http://dx.doi.org/10.1038/s41589-020-0493-1
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author Italia, James S.
Peeler, Jennifer C.
Hillenbrand, Christen M.
Latour, Christopher
Weerapana, Eranthie
Chatterjee, Abhishek
author_facet Italia, James S.
Peeler, Jennifer C.
Hillenbrand, Christen M.
Latour, Christopher
Weerapana, Eranthie
Chatterjee, Abhishek
author_sort Italia, James S.
collection PubMed
description Tyrosine sulfation is an important post-translational modification found in higher eukaryotes. Here we report an engineered tyrosyl-tRNA synthetase/tRNA pair that co-translationally incorporates O-sulfotyrosine in response to UAG codons in E. coli and mammalian cells. This platform enables recombinant expression of eukaryotic proteins homogeneously sulfated at chosen sites, which was demonstrated by expressing human heparin cofactor II in mammalian cells in different states of sulfation.
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spelling pubmed-75648912020-10-16 Genetically encoded protein sulfation in mammalian cells Italia, James S. Peeler, Jennifer C. Hillenbrand, Christen M. Latour, Christopher Weerapana, Eranthie Chatterjee, Abhishek Nat Chem Biol Article Tyrosine sulfation is an important post-translational modification found in higher eukaryotes. Here we report an engineered tyrosyl-tRNA synthetase/tRNA pair that co-translationally incorporates O-sulfotyrosine in response to UAG codons in E. coli and mammalian cells. This platform enables recombinant expression of eukaryotic proteins homogeneously sulfated at chosen sites, which was demonstrated by expressing human heparin cofactor II in mammalian cells in different states of sulfation. 2020-03-16 2020-04 /pmc/articles/PMC7564891/ /pubmed/32198493 http://dx.doi.org/10.1038/s41589-020-0493-1 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Italia, James S.
Peeler, Jennifer C.
Hillenbrand, Christen M.
Latour, Christopher
Weerapana, Eranthie
Chatterjee, Abhishek
Genetically encoded protein sulfation in mammalian cells
title Genetically encoded protein sulfation in mammalian cells
title_full Genetically encoded protein sulfation in mammalian cells
title_fullStr Genetically encoded protein sulfation in mammalian cells
title_full_unstemmed Genetically encoded protein sulfation in mammalian cells
title_short Genetically encoded protein sulfation in mammalian cells
title_sort genetically encoded protein sulfation in mammalian cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564891/
https://www.ncbi.nlm.nih.gov/pubmed/32198493
http://dx.doi.org/10.1038/s41589-020-0493-1
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AT weerapanaeranthie geneticallyencodedproteinsulfationinmammaliancells
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