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Dictyostelium Cell Fixation: Two Simple Tricks

We share two simple modifications to enhance the fixation and imaging of relatively small, motile, and rounded model cells. These include cell centrifugation and the addition of trace amounts of glutaraldehyde to existing fixation methods. Though they need to be carefully considered in each context,...

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Detalles Bibliográficos
Autores principales: Koonce, Michael, Tikhonenko, Irina, Gräf, Ralph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565140/
https://www.ncbi.nlm.nih.gov/pubmed/32630359
http://dx.doi.org/10.3390/mps3030047