Unexpectedly High Levels of Inverted Re-Insertions Using Paired sgRNAs for Genomic Deletions

Use of dual sgRNAs is a common CRISPR/Cas9-based strategy for the creation of genetic deletions. The ease of screening combined with a rather high rate of success makes this approach a reliable genome engineering procedure. Recently, a number of studies using CRISPR/Cas9 have revealed unwanted large...

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Detalles Bibliográficos
Autores principales: Blayney, Joseph, Foster, Evangeline M., Jagielowicz, Marta, Kreuzer, Mira, Morotti, Matteo, Reglinski, Katharina, Xiao, Julie Huiyuan, Hublitz, Philip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565582/
https://www.ncbi.nlm.nih.gov/pubmed/32751356
http://dx.doi.org/10.3390/mps3030053

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565582/
https://www.ncbi.nlm.nih.gov/pubmed/32751356
http://dx.doi.org/10.3390/mps3030053

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