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Efficient and cost-effective bacterial mRNA sequencing from low input samples through ribosomal RNA depletion

BACKGROUND: RNA sequencing is a powerful approach to quantify the genome-wide distribution of mRNA molecules in a population to gain deeper understanding of cellular functions and phenotypes. However, unlike eukaryotic cells, mRNA sequencing of bacterial samples is more challenging due to the absenc...

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Detalles Bibliográficos
Autores principales: Wangsanuwat, Chatarin, Heom, Kellie A., Liu, Estella, O’Malley, Michelle A., Dey, Siddharth S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565789/
https://www.ncbi.nlm.nih.gov/pubmed/33066726
http://dx.doi.org/10.1186/s12864-020-07134-4