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Thermoneutral Housing Temperature Improves Survival in a Murine Model of Polymicrobial Peritonitis

Regulatory guidelines mandate housing for laboratory mice at temperatures below their thermoneutral zone, creating chronic cold stress. However, increases in housing temperature could alter immune responses. We hypothesized housing mice at temperatures within their thermoneutral zone would improve s...

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Autores principales: Carpenter, Kelsey C., Zhou, Yesen, Hakenjos, John M., Fry, Christopher D., Nemzek, Jean A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7566308/
https://www.ncbi.nlm.nih.gov/pubmed/32433210
http://dx.doi.org/10.1097/SHK.0000000000001551
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author Carpenter, Kelsey C.
Zhou, Yesen
Hakenjos, John M.
Fry, Christopher D.
Nemzek, Jean A.
author_facet Carpenter, Kelsey C.
Zhou, Yesen
Hakenjos, John M.
Fry, Christopher D.
Nemzek, Jean A.
author_sort Carpenter, Kelsey C.
collection PubMed
description Regulatory guidelines mandate housing for laboratory mice at temperatures below their thermoneutral zone, creating chronic cold stress. However, increases in housing temperature could alter immune responses. We hypothesized housing mice at temperatures within their thermoneutral zone would improve sepsis survival and alter immune responses. Male C57BL/6 mice were housed at 22°C or 30°C after cecal ligation and puncture (CLP) for 10 days. Survival of mice housed at 30°C (78%) after CLP was significantly increased compared with mice housed at 22°C (40%). Experimental groups were repeated with mice euthanized at 0, 12, 24, and 48 h post-surgery to examine select immune parameters. Raising housing temperature minimally altered systemic, peritoneal, or splenic cell counts. However, IL-6 levels in plasma and peritoneal lavage fluid were significantly lower at 12 h post-surgery in mice housed at 30°C compared with 22°C. Bacterial colony counts from peritoneal lavage fluid were significantly lower in mice housed at 30°C and in vivo studies suggested this was the result of increased phagocytosis by neutrophils. As previously demonstrated, adoptive transfer of fibrocytes significantly increased sepsis survival compared with saline at 22°C. However, there was no additive effect when adoptive transfer was performed at 30°C. Overall, the results demonstrated that thermoneutral housing improves survival after CLP by increasing local phagocytic activity and technical revisions may be necessary to standardize the severity of the model across different housing temperatures. These findings stress the pronounced impact housing temperature has on the CLP model and the importance of reporting housing temperature.
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spelling pubmed-75663082020-10-29 Thermoneutral Housing Temperature Improves Survival in a Murine Model of Polymicrobial Peritonitis Carpenter, Kelsey C. Zhou, Yesen Hakenjos, John M. Fry, Christopher D. Nemzek, Jean A. Shock Basic Science Aspects Regulatory guidelines mandate housing for laboratory mice at temperatures below their thermoneutral zone, creating chronic cold stress. However, increases in housing temperature could alter immune responses. We hypothesized housing mice at temperatures within their thermoneutral zone would improve sepsis survival and alter immune responses. Male C57BL/6 mice were housed at 22°C or 30°C after cecal ligation and puncture (CLP) for 10 days. Survival of mice housed at 30°C (78%) after CLP was significantly increased compared with mice housed at 22°C (40%). Experimental groups were repeated with mice euthanized at 0, 12, 24, and 48 h post-surgery to examine select immune parameters. Raising housing temperature minimally altered systemic, peritoneal, or splenic cell counts. However, IL-6 levels in plasma and peritoneal lavage fluid were significantly lower at 12 h post-surgery in mice housed at 30°C compared with 22°C. Bacterial colony counts from peritoneal lavage fluid were significantly lower in mice housed at 30°C and in vivo studies suggested this was the result of increased phagocytosis by neutrophils. As previously demonstrated, adoptive transfer of fibrocytes significantly increased sepsis survival compared with saline at 22°C. However, there was no additive effect when adoptive transfer was performed at 30°C. Overall, the results demonstrated that thermoneutral housing improves survival after CLP by increasing local phagocytic activity and technical revisions may be necessary to standardize the severity of the model across different housing temperatures. These findings stress the pronounced impact housing temperature has on the CLP model and the importance of reporting housing temperature. Lippincott Williams & Wilkins 2020-11 2020-05-15 /pmc/articles/PMC7566308/ /pubmed/32433210 http://dx.doi.org/10.1097/SHK.0000000000001551 Text en Copyright © 2020 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the Shock Society. This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Basic Science Aspects
Carpenter, Kelsey C.
Zhou, Yesen
Hakenjos, John M.
Fry, Christopher D.
Nemzek, Jean A.
Thermoneutral Housing Temperature Improves Survival in a Murine Model of Polymicrobial Peritonitis
title Thermoneutral Housing Temperature Improves Survival in a Murine Model of Polymicrobial Peritonitis
title_full Thermoneutral Housing Temperature Improves Survival in a Murine Model of Polymicrobial Peritonitis
title_fullStr Thermoneutral Housing Temperature Improves Survival in a Murine Model of Polymicrobial Peritonitis
title_full_unstemmed Thermoneutral Housing Temperature Improves Survival in a Murine Model of Polymicrobial Peritonitis
title_short Thermoneutral Housing Temperature Improves Survival in a Murine Model of Polymicrobial Peritonitis
title_sort thermoneutral housing temperature improves survival in a murine model of polymicrobial peritonitis
topic Basic Science Aspects
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7566308/
https://www.ncbi.nlm.nih.gov/pubmed/32433210
http://dx.doi.org/10.1097/SHK.0000000000001551
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