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High-yield skeletal muscle protein recovery from TRIzol after RNA and DNA extraction

Extraction of DNA, RNA and protein from the same sample would allow for direct comparison of genomic, transcriptomic and proteomic information. Commercially available kits exhibit poor protein yield and the TRIzol(®) reagent produces a protein pellet that is extremely difficult to solubilize. In res...

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Detalles Bibliográficos
Autores principales: Wen, Yuan, Vechetti, Ivan J, Valentino, Taylor R, McCarthy, John J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7566772/
https://www.ncbi.nlm.nih.gov/pubmed/32777951
http://dx.doi.org/10.2144/btn-2020-0083