Protocol for the Generation of Human Pluripotent Reporter Cell Lines Using CRISPR/Cas9

Reporter cell lines based on human pluripotent stem cells (hPSCs) are highly desirable for studying differentiation, lineage tracing, and target cell selection. However, several technical bottlenecks, such as DNA transduction, low homology recombination rate (HDR), and single-cell cloning, have made...

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Detalles Bibliográficos
Autores principales: Zhong, Aaron, Li, Mu, Zhou, Ting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7566848/
https://www.ncbi.nlm.nih.gov/pubmed/33073252
http://dx.doi.org/10.1016/j.xpro.2020.100052

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7566848/
https://www.ncbi.nlm.nih.gov/pubmed/33073252
http://dx.doi.org/10.1016/j.xpro.2020.100052

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