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Protocol for the Generation of Human Pluripotent Reporter Cell Lines Using CRISPR/Cas9
Reporter cell lines based on human pluripotent stem cells (hPSCs) are highly desirable for studying differentiation, lineage tracing, and target cell selection. However, several technical bottlenecks, such as DNA transduction, low homology recombination rate (HDR), and single-cell cloning, have made...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Elsevier
2020
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7566848/ https://www.ncbi.nlm.nih.gov/pubmed/33073252 http://dx.doi.org/10.1016/j.xpro.2020.100052 |