Cargando…

Dissecting the roles of GRK2 and GRK3 in μ-opioid receptor internalization and β-arrestin2 recruitment using CRISPR/Cas9-edited HEK293 cells

Most G protein-coupled receptors (GPCRs) recruit β-arrestins and internalize upon agonist stimulation. For the μ-opioid receptor (μ-OR), this process has been linked to development of opioid tolerance. GPCR kinases (GRKs), particularly GRK2 and GRK3, have been shown to be important for μ-OR recruitm...

Descripción completa

Detalles Bibliográficos
Autores principales:	Møller, Thor C., Pedersen, Mie F., van Senten, Jeffrey R., Seiersen, Sofie D., Mathiesen, Jesper M., Bouvier, Michel, Bräuner-Osborne, Hans
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7567791/ https://www.ncbi.nlm.nih.gov/pubmed/33060647 http://dx.doi.org/10.1038/s41598-020-73674-0

Ejemplares similares

Use of CRISPR/Cas9-edited HEK293 cells reveals that both conventional and novel protein kinase C isozymes are involved in mGlu(5a) receptor internalization
por: van Senten, Jeffrey R., et al.
Publicado: (2022)

Regulation of Constitutive GPR3 Signaling and Surface Localization by GRK2 and β-arrestin-2 Overexpression in HEK293 Cells
por: Lowther, Katie M., et al.
Publicado: (2013)

Double life: How GRK2 and β-arrestin signaling participate in diseases
por: Zhai, Ruxu, et al.
Publicado: (2022)

Trafficking GRK2: Cellular and Metabolic consequences of GRK2 subcellular localization.
por: Sorriento, Daniela, et al.
Publicado: (2014)

The β-blocker Nebivolol Is a GRK/β-arrestin Biased Agonist
por: Erickson, Catherine E., et al.
Publicado: (2013)

Antagonistic Roles of GRK2 and GRK5 in Cardiac Aldosterone Signaling Reveal GRK5-Mediated Cardioprotection via Mineralocorticoid Receptor Inhibition
por: Maning, Jennifer, et al.
Publicado: (2020)

ACKR3–arrestin2/3 complexes reveal molecular consequences of GRK-dependent barcoding
por: Chen, Qiuyan, et al.
Publicado: (2023)

ACKR4 Recruits GRK3 Prior to β-Arrestins but Can Scavenge Chemokines in the Absence of β-Arrestins
por: Matti, Christoph, et al.
Publicado: (2020)

Suitability of GRK Antibodies for Individual Detection and Quantification of GRK Isoforms in Western Blots
por: Reichel, Mona, et al.
Publicado: (2022)

GRK Mediates μ-Opioid Receptor Plasma Membrane Reorganization
por: Gondin, Arisbel B., et al.
Publicado: (2019)

Isoprenaline and salbutamol inhibit pyroptosis and promote mitochondrial biogenesis in arthritic chondrocytes by downregulating β-arrestin and GRK2
por: Ajmal, Iqra, et al.
Publicado: (2022)

Effect of the 5-HT(4) receptor agonist tegaserod on the expression of GRK2 and GRK6 in the rat gastrointestinal tract
por: Nedi, Teshome, et al.
Publicado: (2018)

Gαs is dispensable for β-arrestin coupling but dictates GRK selectivity and is predominant for gene expression regulation by β2-adrenergic receptor
por: Burghi, Valeria, et al.
Publicado: (2023)

Disentangling bias between G(q), GRK2, and arrestin3 recruitment to the M(3) muscarinic acetylcholine receptor
por: Flöser, Anja, et al.
Publicado: (2021)

G Protein-Coupled Receptor Kinase 2 (GRK2) and 5 (GRK5) Exhibit Selective Phosphorylation of the Neurotensin Receptor in Vitro
por: Inagaki, Sayaka, et al.
Publicado: (2015)

Ubiquitination of GRK2 Is Required for the β-Arrestin-Biased Signaling Pathway of Dopamine D2 Receptors to Activate ERK Kinases
por: Liu, Haiping, et al.
Publicado: (2023)

Co-Expression of GRK2 Reveals a Novel Conformational State of the µ-Opioid Receptor
por: Nickolls, Sarah A., et al.
Publicado: (2013)

Effects of the Dopamine D2 Allosteric Modulator, PAOPA, on the Expression of GRK2, Arrestin-3, ERK1/2, and on Receptor Internalization
por: Basu, Dipannita, et al.
Publicado: (2013)

Canonical and Non-Canonical Roles of GRK2 in Lymphocytes
por: Cheng, Jing, et al.
Publicado: (2021)

CXCR7: a β-arrestin-biased receptor that potentiates cell migration and recruits β-arrestin2 exclusively through Gβγ subunits and GRK2
por: Nguyen, Huong Thi, et al.
Publicado: (2020)

Grk1b and Grk7a Both Contribute to the Recovery of the Isolated Cone Photoresponse in Larval Zebrafish
por: Chrispell, Jared D., et al.
Publicado: (2018)

Quantitative Modeling of GRK-Mediated β2AR Regulation
por: Vayttaden, Sharat J., et al.
Publicado: (2010)

An essential role for Grk2 in Hedgehog signalling downstream of Smoothened
por: Zhao, Zhonghua, et al.
Publicado: (2016)

The Metabolic Role of GRK2 in Insulin Resistance and Associated Conditions
por: Sorriento, Daniela, et al.
Publicado: (2021)

Cardiac GRK2 and the Communicative Axis Between Heart and Fat
por: Hill, Bradford G.
Publicado: (2022)

The GIP Receptor Displays Higher Basal Activity than the GLP-1 Receptor but Does Not Recruit GRK2 or Arrestin3 Effectively
por: Al-Sabah, Suleiman, et al.
Publicado: (2014)

Heterotrimeric Gq proteins act as a switch for GRK5/6 selectivity underlying β-arrestin transducer bias
por: Kawakami, Kouki, et al.
Publicado: (2022)

Correction: Maning et al. Antagonistic Roles of GRK2 and GRK5 in Cardiac Aldosterone Signaling Reveal GRK5-Mediated Cardioprotection via Mineralocorticoid Receptor Inhibition. Int. J. Mol. Sci. 2020, 21, 2868
por: Maning, Jennifer, et al.
Publicado: (2021)

GRK2 Enforces Androgen Receptor-Dependence in the Prostate and Prostate Tumors
por: Adler, Adam J., et al.
Publicado: (2020)

Dual role of GRK5 in cancer development and progression
por: Gambardella, J, et al.
Publicado: (2016)

Targeting GRK5 for Treating Chronic Degenerative Diseases
por: Marzano, Federica, et al.
Publicado: (2021)

GRK3 as a Prognosis Biomarker in Gastric Cancer
por: Fang, Chia-Lang, et al.
Publicado: (2022)

Differential Regulation of GPCRs—Are GRK Expression Levels the Key?
por: Matthees, Edda S. F., et al.
Publicado: (2021)

GRK2 regulates group 2 innate lymphoid cell mobilization in sepsis
por: Lai, Dengming, et al.
Publicado: (2022)

Impaired Recruitment of Grk6 and β-Arrestin2 Causes Delayed Internalization and Desensitization of a WHIM Syndrome-Associated CXCR4 Mutant Receptor
por: McCormick, Peter J., et al.
Publicado: (2009)

Grk7 but not Grk1 undergoes cAMP-dependent phosphorylation in zebrafish cone photoreceptors and mediates cone photoresponse recovery to elevated cAMP
por: Chrispell, Jared D., et al.
Publicado: (2022)

Roles of GRK and PDE4 Activities in the Regulation of β(2) Adrenergic Signaling
por: Xin, Wenkuan, et al.
Publicado: (2008)

Antidiabetic and Cardioprotective Effects of Pharmacological Inhibition of GRK2 in db/db Mice
por: Cipolletta, Ersilia, et al.
Publicado: (2019)

Suppression of GRK2 expression reduces endothelial dysfunction by restoring glucose homeostasis
por: Taguchi, Kumiko, et al.
Publicado: (2017)

Degradation of GRK2 and AKT is an early and detrimental event in myocardial ischemia/reperfusion
por: Penela, Petronila, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_4s9dh65le9p9tve19nf606mqtm