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Converting Escherichia coli MG1655 into a chemical overproducer through inactivating defense system against exogenous DNA
Escherichia coli strain K-12 MG1655 has been proposed as an appropriate host strain for industrial production. However, the direct application of this strain suffers from the transformation inefficiency and plasmid instability. Herein, we conducted genetic modifications at a serial of loci of MG1655...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7568196/ https://www.ncbi.nlm.nih.gov/pubmed/33102829 http://dx.doi.org/10.1016/j.synbio.2020.10.005 |
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author | Wang, Jingge Huang, Chaoyong Guo, Kai Ma, Lianjie Meng, Xiangyu Wang, Ning Huo, Yi-Xin |
author_facet | Wang, Jingge Huang, Chaoyong Guo, Kai Ma, Lianjie Meng, Xiangyu Wang, Ning Huo, Yi-Xin |
author_sort | Wang, Jingge |
collection | PubMed |
description | Escherichia coli strain K-12 MG1655 has been proposed as an appropriate host strain for industrial production. However, the direct application of this strain suffers from the transformation inefficiency and plasmid instability. Herein, we conducted genetic modifications at a serial of loci of MG1655 genome, generating a robust and universal host strain JW128 with higher transformation efficiency and plasmid stability that can be used to efficiently produce desired chemicals after introducing the corresponding synthetic pathways. Using JW128 as the host, the titer of isobutanol reached 5.76 g/L in shake-flask fermentation, and the titer of lycopene reached 1.91 g/L in test-tube fermentation, 40-fold and 5-fold higher than that of original MG1655, respectively. These results demonstrated JW128 is a promising chassis for high-level production of value-added chemicals. |
format | Online Article Text |
id | pubmed-7568196 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | KeAi Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-75681962020-10-23 Converting Escherichia coli MG1655 into a chemical overproducer through inactivating defense system against exogenous DNA Wang, Jingge Huang, Chaoyong Guo, Kai Ma, Lianjie Meng, Xiangyu Wang, Ning Huo, Yi-Xin Synth Syst Biotechnol Article Escherichia coli strain K-12 MG1655 has been proposed as an appropriate host strain for industrial production. However, the direct application of this strain suffers from the transformation inefficiency and plasmid instability. Herein, we conducted genetic modifications at a serial of loci of MG1655 genome, generating a robust and universal host strain JW128 with higher transformation efficiency and plasmid stability that can be used to efficiently produce desired chemicals after introducing the corresponding synthetic pathways. Using JW128 as the host, the titer of isobutanol reached 5.76 g/L in shake-flask fermentation, and the titer of lycopene reached 1.91 g/L in test-tube fermentation, 40-fold and 5-fold higher than that of original MG1655, respectively. These results demonstrated JW128 is a promising chassis for high-level production of value-added chemicals. KeAi Publishing 2020-10-15 /pmc/articles/PMC7568196/ /pubmed/33102829 http://dx.doi.org/10.1016/j.synbio.2020.10.005 Text en © 2020 Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Wang, Jingge Huang, Chaoyong Guo, Kai Ma, Lianjie Meng, Xiangyu Wang, Ning Huo, Yi-Xin Converting Escherichia coli MG1655 into a chemical overproducer through inactivating defense system against exogenous DNA |
title | Converting Escherichia coli MG1655 into a chemical overproducer through inactivating defense system against exogenous DNA |
title_full | Converting Escherichia coli MG1655 into a chemical overproducer through inactivating defense system against exogenous DNA |
title_fullStr | Converting Escherichia coli MG1655 into a chemical overproducer through inactivating defense system against exogenous DNA |
title_full_unstemmed | Converting Escherichia coli MG1655 into a chemical overproducer through inactivating defense system against exogenous DNA |
title_short | Converting Escherichia coli MG1655 into a chemical overproducer through inactivating defense system against exogenous DNA |
title_sort | converting escherichia coli mg1655 into a chemical overproducer through inactivating defense system against exogenous dna |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7568196/ https://www.ncbi.nlm.nih.gov/pubmed/33102829 http://dx.doi.org/10.1016/j.synbio.2020.10.005 |
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