Effects of Exogenous Melatonin on MAM Induced Lung Injury and Lung Development in Mice Offspring

BACKGROUND: Melatonin as an antioxidant agent can have an effective role in lung development. In this study, the effect of melatonin administration on lung injury in the neonate mice was assessed. MATERIALS AND METHODS: Lung injury was induced by two injections of 15 mg/kg methylazoxymethanol (MAM)...

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Autores principales: Azizi, Maryam, Pasbakhsh, Parichehr, Sadr, Makan, Mokhtari, Tahmineh, Pourabdollah, Mihan, Nadji, Seyed Alireza, Ragerdi Kashani, Iraj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Research Institute of Tuberculosis and Lung Disease 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7569496/
https://www.ncbi.nlm.nih.gov/pubmed/33101434
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author Azizi, Maryam
Pasbakhsh, Parichehr
Sadr, Makan
Mokhtari, Tahmineh
Pourabdollah, Mihan
Nadji, Seyed Alireza
Ragerdi Kashani, Iraj
author_facet Azizi, Maryam
Pasbakhsh, Parichehr
Sadr, Makan
Mokhtari, Tahmineh
Pourabdollah, Mihan
Nadji, Seyed Alireza
Ragerdi Kashani, Iraj
author_sort Azizi, Maryam
collection PubMed
description BACKGROUND: Melatonin as an antioxidant agent can have an effective role in lung development. In this study, the effect of melatonin administration on lung injury in the neonate mice was assessed. MATERIALS AND METHODS: Lung injury was induced by two injections of 15 mg/kg methylazoxymethanol (MAM) on gestational day 15 (E15). Pregnant BALB/c mice were randomly divided into five groups: Control (CO), Melatonin (MEL), Luzindole (Luz), MAM, and MAM+MEL. Melatonin and luzindole were intra-peritoneally injected at a dose of 10 mg/kg (from E15 until delivery). Histopathological changes including: hemorrhage, neutrophils infiltration and fibrosis in the neonate lung were studied by hematoxylin and eosin (H&E) and Masson’s Trichrome staining. Alveolarization and alveolar wall thickness were measured. RESULTS: In histological examination, hemorrhage, neutrophils infiltration and fibrosis were seen in the MAM and Luz groups; however, these injuries were attenuated in the MAM plus melatonin group. Significant reduction of alveolarization was recorded in the MAM and Luz groups compared to the control group, while the alveolar wall thickness was significantly increased in these groups compared to control group. CONCLUSION: Administration of exogenous melatonin in pregnant mice could have a protective effect on the pulmonary development of neonates and could decrease lung injury in neonate mice.
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spelling pubmed-75694962020-10-22 Effects of Exogenous Melatonin on MAM Induced Lung Injury and Lung Development in Mice Offspring Azizi, Maryam Pasbakhsh, Parichehr Sadr, Makan Mokhtari, Tahmineh Pourabdollah, Mihan Nadji, Seyed Alireza Ragerdi Kashani, Iraj Tanaffos Original Article BACKGROUND: Melatonin as an antioxidant agent can have an effective role in lung development. In this study, the effect of melatonin administration on lung injury in the neonate mice was assessed. MATERIALS AND METHODS: Lung injury was induced by two injections of 15 mg/kg methylazoxymethanol (MAM) on gestational day 15 (E15). Pregnant BALB/c mice were randomly divided into five groups: Control (CO), Melatonin (MEL), Luzindole (Luz), MAM, and MAM+MEL. Melatonin and luzindole were intra-peritoneally injected at a dose of 10 mg/kg (from E15 until delivery). Histopathological changes including: hemorrhage, neutrophils infiltration and fibrosis in the neonate lung were studied by hematoxylin and eosin (H&E) and Masson’s Trichrome staining. Alveolarization and alveolar wall thickness were measured. RESULTS: In histological examination, hemorrhage, neutrophils infiltration and fibrosis were seen in the MAM and Luz groups; however, these injuries were attenuated in the MAM plus melatonin group. Significant reduction of alveolarization was recorded in the MAM and Luz groups compared to the control group, while the alveolar wall thickness was significantly increased in these groups compared to control group. CONCLUSION: Administration of exogenous melatonin in pregnant mice could have a protective effect on the pulmonary development of neonates and could decrease lung injury in neonate mice. National Research Institute of Tuberculosis and Lung Disease 2020-01 /pmc/articles/PMC7569496/ /pubmed/33101434 Text en Copyright© 2020 National Research Institute of Tuberculosis and Lung Disease http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Azizi, Maryam
Pasbakhsh, Parichehr
Sadr, Makan
Mokhtari, Tahmineh
Pourabdollah, Mihan
Nadji, Seyed Alireza
Ragerdi Kashani, Iraj
Effects of Exogenous Melatonin on MAM Induced Lung Injury and Lung Development in Mice Offspring
title Effects of Exogenous Melatonin on MAM Induced Lung Injury and Lung Development in Mice Offspring
title_full Effects of Exogenous Melatonin on MAM Induced Lung Injury and Lung Development in Mice Offspring
title_fullStr Effects of Exogenous Melatonin on MAM Induced Lung Injury and Lung Development in Mice Offspring
title_full_unstemmed Effects of Exogenous Melatonin on MAM Induced Lung Injury and Lung Development in Mice Offspring
title_short Effects of Exogenous Melatonin on MAM Induced Lung Injury and Lung Development in Mice Offspring
title_sort effects of exogenous melatonin on mam induced lung injury and lung development in mice offspring
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7569496/
https://www.ncbi.nlm.nih.gov/pubmed/33101434
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