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Resilience of Aspergillus westerdijkiae Strains to Interacting Climate-Related Abiotic Factors: Effects on Growth and Ochratoxin A Production on Coffee-Based Medium and in Stored Coffee

We examined the resilience of strains of Aspergillus westerdijkiae in terms of growth and ochratoxin A (OTA) production in relation to: (a) two-way interacting climate-related abiotic factors of water activity (a(w), 0.99–0.90) × temperature (25–37 °C) on green coffee and roasted coffee-based media;...

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Autores principales: Akbar, Asya, Medina, Angel, Magan, Naresh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7569885/
https://www.ncbi.nlm.nih.gov/pubmed/32825420
http://dx.doi.org/10.3390/microorganisms8091268
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author Akbar, Asya
Medina, Angel
Magan, Naresh
author_facet Akbar, Asya
Medina, Angel
Magan, Naresh
author_sort Akbar, Asya
collection PubMed
description We examined the resilience of strains of Aspergillus westerdijkiae in terms of growth and ochratoxin A (OTA) production in relation to: (a) two-way interacting climate-related abiotic factors of water activity (a(w), 0.99–0.90) × temperature (25–37 °C) on green coffee and roasted coffee-based media; (b) three-way climate-related abiotic factors (temperature, 30 vs. 35 °C; water stress, 0.98–0.90 a(w); CO(2), 400 vs. 1000 ppm) on growth and OTA production on a 6% green coffee extract-based matrix; and (c) the effect of three-way climate-related abiotic factors on OTA production in stored green coffee beans. Four strains of A. westerdijkiae grew equally well on green or roasted coffee-based media with optimum 0.98 a(w) and 25–30 °C. Growth was significantly slower on roasted than green coffee-based media at 35 °C, regardless of a(w) level. Interestingly, on green coffee-based media OTA production was optimum at 0.98–0.95 a(w) and 30 °C. However, on roasted coffee-based media very little OTA was produced. Three-way climate-related abiotic factors were examined on two of these strains. These interacting factors significantly reduced growth of the A. westerdijkiae strains, especially at 35 °C × 1000 ppm CO(2) and all a(w) levels when compared to 30 °C. At 35 °C × 1000 ppm CO(2) there was some stimulation of OTA production by the two A. westerdijkiae strains, especially under water stress. In stored green coffee beans optimum OTA was produced at 0.95–0.97 a(w)/30 °C. In elevated CO(2) and 35 °C, OTA production was stimulated at 0.95–0.90 a(w).
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spelling pubmed-75698852020-10-29 Resilience of Aspergillus westerdijkiae Strains to Interacting Climate-Related Abiotic Factors: Effects on Growth and Ochratoxin A Production on Coffee-Based Medium and in Stored Coffee Akbar, Asya Medina, Angel Magan, Naresh Microorganisms Article We examined the resilience of strains of Aspergillus westerdijkiae in terms of growth and ochratoxin A (OTA) production in relation to: (a) two-way interacting climate-related abiotic factors of water activity (a(w), 0.99–0.90) × temperature (25–37 °C) on green coffee and roasted coffee-based media; (b) three-way climate-related abiotic factors (temperature, 30 vs. 35 °C; water stress, 0.98–0.90 a(w); CO(2), 400 vs. 1000 ppm) on growth and OTA production on a 6% green coffee extract-based matrix; and (c) the effect of three-way climate-related abiotic factors on OTA production in stored green coffee beans. Four strains of A. westerdijkiae grew equally well on green or roasted coffee-based media with optimum 0.98 a(w) and 25–30 °C. Growth was significantly slower on roasted than green coffee-based media at 35 °C, regardless of a(w) level. Interestingly, on green coffee-based media OTA production was optimum at 0.98–0.95 a(w) and 30 °C. However, on roasted coffee-based media very little OTA was produced. Three-way climate-related abiotic factors were examined on two of these strains. These interacting factors significantly reduced growth of the A. westerdijkiae strains, especially at 35 °C × 1000 ppm CO(2) and all a(w) levels when compared to 30 °C. At 35 °C × 1000 ppm CO(2) there was some stimulation of OTA production by the two A. westerdijkiae strains, especially under water stress. In stored green coffee beans optimum OTA was produced at 0.95–0.97 a(w)/30 °C. In elevated CO(2) and 35 °C, OTA production was stimulated at 0.95–0.90 a(w). MDPI 2020-08-20 /pmc/articles/PMC7569885/ /pubmed/32825420 http://dx.doi.org/10.3390/microorganisms8091268 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Akbar, Asya
Medina, Angel
Magan, Naresh
Resilience of Aspergillus westerdijkiae Strains to Interacting Climate-Related Abiotic Factors: Effects on Growth and Ochratoxin A Production on Coffee-Based Medium and in Stored Coffee
title Resilience of Aspergillus westerdijkiae Strains to Interacting Climate-Related Abiotic Factors: Effects on Growth and Ochratoxin A Production on Coffee-Based Medium and in Stored Coffee
title_full Resilience of Aspergillus westerdijkiae Strains to Interacting Climate-Related Abiotic Factors: Effects on Growth and Ochratoxin A Production on Coffee-Based Medium and in Stored Coffee
title_fullStr Resilience of Aspergillus westerdijkiae Strains to Interacting Climate-Related Abiotic Factors: Effects on Growth and Ochratoxin A Production on Coffee-Based Medium and in Stored Coffee
title_full_unstemmed Resilience of Aspergillus westerdijkiae Strains to Interacting Climate-Related Abiotic Factors: Effects on Growth and Ochratoxin A Production on Coffee-Based Medium and in Stored Coffee
title_short Resilience of Aspergillus westerdijkiae Strains to Interacting Climate-Related Abiotic Factors: Effects on Growth and Ochratoxin A Production on Coffee-Based Medium and in Stored Coffee
title_sort resilience of aspergillus westerdijkiae strains to interacting climate-related abiotic factors: effects on growth and ochratoxin a production on coffee-based medium and in stored coffee
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7569885/
https://www.ncbi.nlm.nih.gov/pubmed/32825420
http://dx.doi.org/10.3390/microorganisms8091268
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