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Simple innovative adaptor to improve genome walking with convenient PCR
BACKGROUND: Various polymerase chain reaction (PCR)-based methods have been applied for the development of genome walking (GW) technique. These methods which could be based on the application of restriction enzymes or primers have various efficiencies to identify the unknown nucleotide sequences. Th...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7575660/ https://www.ncbi.nlm.nih.gov/pubmed/33083895 http://dx.doi.org/10.1186/s43141-020-00082-2 |