Cargando…
Protocol for Single-Molecule Fluorescence In Situ Hybridization for Intact Pancreatic Tissue
We describe an optimized smFISH protocol for the intact pancreas. The protocol is adapted from Lyubimova et al. (2013), a generic tissue smFISH protocol that works for most tissues but not the pancreas. The main changes implemented include increasing the period of mRNA denaturation from 5 min to at...
| Autores principales: | , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2020
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580104/ https://www.ncbi.nlm.nih.gov/pubmed/33111069 http://dx.doi.org/10.1016/j.xpro.2019.100007 |