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Highly Efficient CRISPR-Associated Protein 9 Ribonucleoprotein-Based Genome Editing in Euglena gracilis

Euglena gracilis, a unicellular phytoflagellate microalga, is a promising biomaterial for foods, feeds, and biofuels. However, targeted mutagenesis in this species has been a long-standing challenge. We recently developed a transgene-free, highly efficient, genome editing method for E. gracilis usin...

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Detalles Bibliográficos
Autores principales: Nomura, Toshihisa, Yoshikawa, Mizuki, Suzuki, Kengo, Mochida, Keiichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580193/
https://www.ncbi.nlm.nih.gov/pubmed/33111076
http://dx.doi.org/10.1016/j.xpro.2020.100023