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Highly Efficient CRISPR-Associated Protein 9 Ribonucleoprotein-Based Genome Editing in Euglena gracilis
Euglena gracilis, a unicellular phytoflagellate microalga, is a promising biomaterial for foods, feeds, and biofuels. However, targeted mutagenesis in this species has been a long-standing challenge. We recently developed a transgene-free, highly efficient, genome editing method for E. gracilis usin...
Autores principales: | Nomura, Toshihisa, Yoshikawa, Mizuki, Suzuki, Kengo, Mochida, Keiichi |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580193/ https://www.ncbi.nlm.nih.gov/pubmed/33111076 http://dx.doi.org/10.1016/j.xpro.2020.100023 |
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