Rapid Testing of CRISPR Nucleases and Guide RNAs in an E. coli Cell-Free Transcription-Translation System

We present a protocol to rapidly test DNA binding and cleavage activity by CRISPR nucleases using cell-free transcription-translation (TXTL). Nuclease activity is assessed by adding DNA encoding a nuclease, a guide RNA, and a targeted reporter to a TXTL reaction and by measuring the fluorescence for...

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Detalles Bibliográficos
Autores principales: Marshall, Ryan, Beisel, Chase L., Noireaux, Vincent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580202/
https://www.ncbi.nlm.nih.gov/pubmed/33111065
http://dx.doi.org/10.1016/j.xpro.2019.100003

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580202/
https://www.ncbi.nlm.nih.gov/pubmed/33111065
http://dx.doi.org/10.1016/j.xpro.2019.100003

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