Establishment and Use of Patient-Derived Xenograft Models for Drug Testing in Head and Neck Squamous Cell Carcinoma

This protocol provides the steps required for the establishment of patient-derived xenograft (PDX) tumors for head and neck squamous cell carcinomas (HNSCCs) and their utility in examining drug responses. PDXs recapitulate the heterogeneity observed in the corresponding human tumors, which makes the...

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Autores principales: Karamboulas, Christina, Meens, Jalna, Ailles, Laurie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580210/
https://www.ncbi.nlm.nih.gov/pubmed/33111077
http://dx.doi.org/10.1016/j.xpro.2020.100024
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author Karamboulas, Christina
Meens, Jalna
Ailles, Laurie
author_facet Karamboulas, Christina
Meens, Jalna
Ailles, Laurie
author_sort Karamboulas, Christina
collection PubMed
description This protocol provides the steps required for the establishment of patient-derived xenograft (PDX) tumors for head and neck squamous cell carcinomas (HNSCCs) and their utility in examining drug responses. PDXs recapitulate the heterogeneity observed in the corresponding human tumors, which makes them an ideal pre-clinical model system. This protocol outlines the detailed steps required for (1) the generation of HNSCC-PDXs, (2) the processing of tumor tissues, and (3) the expansion of PDX models into cohorts for (4) drug testing. For complete details on the use and execution of this protocol please refer to Karamboulas et al. (2018).
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spelling pubmed-75802102020-10-26 Establishment and Use of Patient-Derived Xenograft Models for Drug Testing in Head and Neck Squamous Cell Carcinoma Karamboulas, Christina Meens, Jalna Ailles, Laurie STAR Protoc Protocol This protocol provides the steps required for the establishment of patient-derived xenograft (PDX) tumors for head and neck squamous cell carcinomas (HNSCCs) and their utility in examining drug responses. PDXs recapitulate the heterogeneity observed in the corresponding human tumors, which makes them an ideal pre-clinical model system. This protocol outlines the detailed steps required for (1) the generation of HNSCC-PDXs, (2) the processing of tumor tissues, and (3) the expansion of PDX models into cohorts for (4) drug testing. For complete details on the use and execution of this protocol please refer to Karamboulas et al. (2018). Elsevier 2020-06-03 /pmc/articles/PMC7580210/ /pubmed/33111077 http://dx.doi.org/10.1016/j.xpro.2020.100024 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Karamboulas, Christina
Meens, Jalna
Ailles, Laurie
Establishment and Use of Patient-Derived Xenograft Models for Drug Testing in Head and Neck Squamous Cell Carcinoma
title Establishment and Use of Patient-Derived Xenograft Models for Drug Testing in Head and Neck Squamous Cell Carcinoma
title_full Establishment and Use of Patient-Derived Xenograft Models for Drug Testing in Head and Neck Squamous Cell Carcinoma
title_fullStr Establishment and Use of Patient-Derived Xenograft Models for Drug Testing in Head and Neck Squamous Cell Carcinoma
title_full_unstemmed Establishment and Use of Patient-Derived Xenograft Models for Drug Testing in Head and Neck Squamous Cell Carcinoma
title_short Establishment and Use of Patient-Derived Xenograft Models for Drug Testing in Head and Neck Squamous Cell Carcinoma
title_sort establishment and use of patient-derived xenograft models for drug testing in head and neck squamous cell carcinoma
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580210/
https://www.ncbi.nlm.nih.gov/pubmed/33111077
http://dx.doi.org/10.1016/j.xpro.2020.100024
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