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Repurposing the Native Type I-F CRISPR-Cas System in Pseudomonas aeruginosa for Genome Editing
Repurposing the broadly distributed native CRISPR-Cas systems in prokaryotes for genome editing is emerging as a new strategy for genetic manipulations. We recently reported the establishment of a single plasmid-mediated, one-step genome-editing technique in a multidrug-resistant genotype of the opp...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580218/ https://www.ncbi.nlm.nih.gov/pubmed/33111087 http://dx.doi.org/10.1016/j.xpro.2020.100039 |