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Protocol for Quantifying Zinc Flux in Cultured Cells using Fluorescent Indicators

Zinc (Zn(2+)) plays a vital role in the functioning of the cell. Cells have influx and efflux zinc transporters to regulate the levels of Zn(2+) in the cytoplasm and organellar compartments to maintain homeostasis. We present a protocol to measure changes in cellular zinc concentrations using either...

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Detalles Bibliográficos
Autores principales: Ali, Saima, Cuajungco, Math P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580219/
https://www.ncbi.nlm.nih.gov/pubmed/33111096
http://dx.doi.org/10.1016/j.xpro.2020.100050