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A Protocol to Engineer Bacteriophages for Live-Cell Imaging of Bacterial Prophage Induction Inside Mammalian Cells

The gut microbiome is dominated by lysogens, bacteria that carry bacterial viruses (phages). Uncovering the function of phages in the microbiome and observing interactions between phages, bacteria, and mammalian cells in real time in specific cell types are limited by the difficulty of engineering f...

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Detalles Bibliográficos
Autores principales: Bodner, Katie, Melkonian, Arin L., Covert, Markus W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580223/
https://www.ncbi.nlm.nih.gov/pubmed/33111117
http://dx.doi.org/10.1016/j.xpro.2020.100084