An Optimized Protocol for the Generation of Midbrain Dopamine Neurons under Defined Conditions

Here, we describe a xeno-free, feeder-free, and chemically defined protocol for the generation of ventral midbrain dopaminergic (vmDA) progenitors from human pluripotent stem cells (hPSCs). This simple-to-follow protocol results in high yields of cryopreservable dopamine neurons across multiple hPSC...

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Detalles Bibliográficos
Autores principales: Gantner, Carlos W., Cota-Coronado, Agustín, Thompson, Lachlan H., Parish, Clare L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580226/
https://www.ncbi.nlm.nih.gov/pubmed/33111103
http://dx.doi.org/10.1016/j.xpro.2020.100065

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580226/
https://www.ncbi.nlm.nih.gov/pubmed/33111103
http://dx.doi.org/10.1016/j.xpro.2020.100065

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