Generation of Sequencing Libraries for Structural Analysis of Bacterial 5′ UTRs

The structure of 5′ untranslated regions (5′ UTRs) of bacterial mRNAs often determines the fate of the transcripts. Using a dimethyl sulfate mutational profiling with sequencing (DMS-MaPseq) approach, we developed a protocol to generate sequence libraries to determine the base-pairing status of aden...

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Detalles Bibliográficos
Autores principales: Ignatov, Dmitriy, Vaitkevicius, Karolis, Johansson, Jörgen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580233/
https://www.ncbi.nlm.nih.gov/pubmed/33111092
http://dx.doi.org/10.1016/j.xpro.2020.100046

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580233/
https://www.ncbi.nlm.nih.gov/pubmed/33111092
http://dx.doi.org/10.1016/j.xpro.2020.100046

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