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Generation of Sequencing Libraries for Structural Analysis of Bacterial 5′ UTRs
The structure of 5′ untranslated regions (5′ UTRs) of bacterial mRNAs often determines the fate of the transcripts. Using a dimethyl sulfate mutational profiling with sequencing (DMS-MaPseq) approach, we developed a protocol to generate sequence libraries to determine the base-pairing status of aden...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580233/ https://www.ncbi.nlm.nih.gov/pubmed/33111092 http://dx.doi.org/10.1016/j.xpro.2020.100046 |