Imaging of Telomerase RNA by Single-Molecule Inexpensive FISH Combined with Immunofluorescence

Fluorescent in situ hybridization (FISH) on the RNA moiety of human telomerase (hTR) with 50-mer probes detects hTR RNA accumulated in Cajal bodies. Using both live-cell imaging and single-molecule inexpensive FISH, our published work revealed that only a fraction of hTR localizes to Cajal bodies, w...

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Detalles Bibliográficos
Autores principales: Querido, Emmanuelle, Sfeir, Agnel, Chartrand, Pascal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580239/
https://www.ncbi.nlm.nih.gov/pubmed/33111129
http://dx.doi.org/10.1016/j.xpro.2020.100104

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580239/
https://www.ncbi.nlm.nih.gov/pubmed/33111129
http://dx.doi.org/10.1016/j.xpro.2020.100104

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