An Optimized Protocol for Proximity Biotinylation in Confluent Epithelial Cell Cultures Using the Peroxidase APEX2

The peroxidase APEX2 has been used widely for proximity biotinylation and subsequent proteomics analyses. However, the poor membrane permeability of the biotin phenol substrate and the inhibitory effect of peroxide on the enzyme’s activity has hampered proximity labeling in certain cell culture syst...

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Detalles Bibliográficos
Autores principales: Tan, Benedict, Peng, Suat, Yatim, Siti Maryam J.M., Gunaratne, Jayantha, Hunziker, Walter, Ludwig, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580243/
https://www.ncbi.nlm.nih.gov/pubmed/33111110
http://dx.doi.org/10.1016/j.xpro.2020.100074

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580243/
https://www.ncbi.nlm.nih.gov/pubmed/33111110
http://dx.doi.org/10.1016/j.xpro.2020.100074

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