Protocol for Titrating Gene Expression Levels in Budding Yeast

The biological phenotype is affected by the level of gene expression. Here, we provide a step-by-step protocol for precisely titrating and quantitatively observing the target gene expression level in budding yeast by manipulating its copy number in the genome. Using this method, we construct various...

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Detalles Bibliográficos
Autores principales: Qu, Yimiao, Jiang, Jun, Yang, Xiaojing, Tang, Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580245/
https://www.ncbi.nlm.nih.gov/pubmed/33111115
http://dx.doi.org/10.1016/j.xpro.2020.100082

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580245/
https://www.ncbi.nlm.nih.gov/pubmed/33111115
http://dx.doi.org/10.1016/j.xpro.2020.100082

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