Protocol for Titrating Gene Expression Levels in Budding Yeast

The biological phenotype is affected by the level of gene expression. Here, we provide a step-by-step protocol for precisely titrating and quantitatively observing the target gene expression level in budding yeast by manipulating its copy number in the genome. Using this method, we construct various...

Descripción completa

Detalles Bibliográficos
Autores principales: Qu, Yimiao, Jiang, Jun, Yang, Xiaojing, Tang, Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580245/
https://www.ncbi.nlm.nih.gov/pubmed/33111115
http://dx.doi.org/10.1016/j.xpro.2020.100082

Ejemplares similares

Ejemplares similares