Effective refolding of a cysteine rich glycoside hydrolase family 19 recombinant chitinase from Streptomyces griseus by reverse dilution and affinity chromatography

Conventional refolding methods are associated with low yields due to misfolding and high aggregation rates or very dilute proteins. In this study, we describe the optimization of the conventional methods of reverse dilution and affinity chromatography for obtaining high yields of a cysteine rich rec...

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Detalles Bibliográficos
Autores principales: Oyeleye, Ayokunmi Omolola, Mohd Yusoff, Siti Faridah, Abd Rahim, Izzah Nadiah, Leow, Adam Thean Chor, Saidi, Noor Baity, Normi, Yahaya M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580917/
https://www.ncbi.nlm.nih.gov/pubmed/33091044
http://dx.doi.org/10.1371/journal.pone.0241074

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580917/
https://www.ncbi.nlm.nih.gov/pubmed/33091044
http://dx.doi.org/10.1371/journal.pone.0241074

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