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Fluorescent secreted bacterial effectors reveal active intravacuolar proliferation of Listeria monocytogenes in epithelial cells

Real-time imaging of bacterial virulence factor dynamics is hampered by the limited number of fluorescent tools suitable for tagging secreted effectors. Here, we demonstrated that the fluorogenic reporter FAST could be used to tag secreted proteins, and we implemented it to monitor infection dynamic...

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Detalles Bibliográficos
Autores principales: Peron-Cane, Caroline, Fernandez, José-Carlos, Leblanc, Julien, Wingertsmann, Laure, Gautier, Arnaud, Desprat, Nicolas, Lebreton, Alice
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580998/
https://www.ncbi.nlm.nih.gov/pubmed/33045003
http://dx.doi.org/10.1371/journal.ppat.1009001