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Fast Purification of Recombinant Monomeric Amyloid-β from E. coli and Amyloid-β-mCherry Aggregates from Mammalian Cells

[Image: see text] The Alzheimer’s disease related peptide, Amyloid-beta (Aβ)1–40 and 1–42, has proven difficult to be purified as a recombinant monomeric protein due its expression in E. coli leading to the formation of insoluble inclusion bodies and its tendency to quickly form insoluble aggregates...

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Detalles Bibliográficos
Autores principales: Stephens, Amberley D., Lu, Meng, Fernandez-Villegas, Ana, Kaminski Schierle, Gabriele S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7581289/
https://www.ncbi.nlm.nih.gov/pubmed/32960567
http://dx.doi.org/10.1021/acschemneuro.0c00300