Application of Short Pre-enrichment, and Double Chemistry Real-Time PCR, Combining Fluorescent Probes and an Intercalating Dye, for Same-Day Detection and Confirmation of Salmonella spp. and Escherichia coli O157 in Ground Beef and Chicken Samples

Molecular methods, particularly those based on real-time PCR (qPCR), have become a popular approach to detect pathogens in food samples. This technique may take advantage of hydrolysis fluorescent probes for increased specificity. Even though suitable, this approach loses the capacity of performing...

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Detalles Bibliográficos
Autores principales: Garrido-Maestu, Alejandro, Azinheiro, Sarah, Roumani, Foteini, Carvalho, Joana, Prado, Marta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7581864/
https://www.ncbi.nlm.nih.gov/pubmed/33162968
http://dx.doi.org/10.3389/fmicb.2020.591041

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7581864/
https://www.ncbi.nlm.nih.gov/pubmed/33162968
http://dx.doi.org/10.3389/fmicb.2020.591041

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