High-throughput continuous-flow microfluidic electroporation of mRNA into primary human T cells for applications in cellular therapy manufacturing

Implementation of gene editing technologies such as CRISPR/Cas9 in the manufacture of novel cell-based therapeutics has the potential to enable highly-targeted, stable, and persistent genome modifications without the use of viral vectors. Electroporation has emerged as a preferred method for deliver...

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Detalles Bibliográficos
Autores principales: Lissandrello, Charles A., Santos, Jose A., Hsi, Peter, Welch, Michaela, Mott, Vienna L., Kim, Ernest S., Chesin, Jordan, Haroutunian, Nerses J., Stoddard, Aaron G., Czarnecki, Andrew, Coppeta, Jonathan R., Freeman, Daniel K., Flusberg, Deborah A., Balestrini, Jenna L., Tandon, Vishal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582186/
https://www.ncbi.nlm.nih.gov/pubmed/33093518
http://dx.doi.org/10.1038/s41598-020-73755-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582186/
https://www.ncbi.nlm.nih.gov/pubmed/33093518
http://dx.doi.org/10.1038/s41598-020-73755-0

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