Cargando…
Gamma Aminobutyric Acid-Enriched Fermented Oyster (Crassostrea gigas) Increases the Length of the Growth Plate on the Proximal Tibia Bone in Sprague-Dawley Rats
Bone growth during childhood and puberty determines an adult’s final stature. Although several prior studies have reported that fermented oyster (FO) consisting of a high amount of gamma aminobutyric acid can be attributed to bone health, there is no research on the efficacy of FO on growth regulati...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582314/ https://www.ncbi.nlm.nih.gov/pubmed/32977643 http://dx.doi.org/10.3390/molecules25194375 |
_version_ | 1783599162589184000 |
---|---|
author | Lee, Hyesook Hwangbo, Hyun Ji, Seon Yeong Kim, Min Yeong Kim, So Young Kim, Da Hye Hong, Su Hyun Lee, Su Jeong Assefa, Freshet Kim, Gi-Young Park, Eui Kyun Park, Joung-Hyun Lee, Bae-Jin Jeon, You-Jin Choi, Yung Hyun |
author_facet | Lee, Hyesook Hwangbo, Hyun Ji, Seon Yeong Kim, Min Yeong Kim, So Young Kim, Da Hye Hong, Su Hyun Lee, Su Jeong Assefa, Freshet Kim, Gi-Young Park, Eui Kyun Park, Joung-Hyun Lee, Bae-Jin Jeon, You-Jin Choi, Yung Hyun |
author_sort | Lee, Hyesook |
collection | PubMed |
description | Bone growth during childhood and puberty determines an adult’s final stature. Although several prior studies have reported that fermented oyster (FO) consisting of a high amount of gamma aminobutyric acid can be attributed to bone health, there is no research on the efficacy of FO on growth regulation and the proximal tibial growth plate. Therefore, in this study, we investigated the effect of FO oral administration on hepatic and serum growth regulator levels and the development of the proximal tibial growth plate in young Sprague-Dawley rats. Both oral administration of FO (FO 100, 100 mg/kg FO and FO 200, 200 mg/kg FO) and subcutaneous injection of recombinant human growth hormone (rhGH, 200 μg/kg of rhGH) for two weeks showed no toxicity. Circulating levels of growth hormone (GH) significantly increased in the FO 200 group. The expression and secretion of insulin-like growth factor-1 (IGF-1) and insulin-like growth factor binding protein-3 (IGFBP-3) were enhanced by FO administration. FO administration promoted the expression of bone morphogenic proteins IGF-1 and IGFBP-3 in the proximal tibial growth plate. This positive effect of FO resulted in incremental growth of the entire plate length by expanding the proliferating and hypertrophic zones in the proximal tibial growth plate. Collectively, our results suggested that oral administration of FO is beneficial for bone health, which may ultimately result in increased height. |
format | Online Article Text |
id | pubmed-7582314 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75823142020-10-28 Gamma Aminobutyric Acid-Enriched Fermented Oyster (Crassostrea gigas) Increases the Length of the Growth Plate on the Proximal Tibia Bone in Sprague-Dawley Rats Lee, Hyesook Hwangbo, Hyun Ji, Seon Yeong Kim, Min Yeong Kim, So Young Kim, Da Hye Hong, Su Hyun Lee, Su Jeong Assefa, Freshet Kim, Gi-Young Park, Eui Kyun Park, Joung-Hyun Lee, Bae-Jin Jeon, You-Jin Choi, Yung Hyun Molecules Article Bone growth during childhood and puberty determines an adult’s final stature. Although several prior studies have reported that fermented oyster (FO) consisting of a high amount of gamma aminobutyric acid can be attributed to bone health, there is no research on the efficacy of FO on growth regulation and the proximal tibial growth plate. Therefore, in this study, we investigated the effect of FO oral administration on hepatic and serum growth regulator levels and the development of the proximal tibial growth plate in young Sprague-Dawley rats. Both oral administration of FO (FO 100, 100 mg/kg FO and FO 200, 200 mg/kg FO) and subcutaneous injection of recombinant human growth hormone (rhGH, 200 μg/kg of rhGH) for two weeks showed no toxicity. Circulating levels of growth hormone (GH) significantly increased in the FO 200 group. The expression and secretion of insulin-like growth factor-1 (IGF-1) and insulin-like growth factor binding protein-3 (IGFBP-3) were enhanced by FO administration. FO administration promoted the expression of bone morphogenic proteins IGF-1 and IGFBP-3 in the proximal tibial growth plate. This positive effect of FO resulted in incremental growth of the entire plate length by expanding the proliferating and hypertrophic zones in the proximal tibial growth plate. Collectively, our results suggested that oral administration of FO is beneficial for bone health, which may ultimately result in increased height. MDPI 2020-09-23 /pmc/articles/PMC7582314/ /pubmed/32977643 http://dx.doi.org/10.3390/molecules25194375 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lee, Hyesook Hwangbo, Hyun Ji, Seon Yeong Kim, Min Yeong Kim, So Young Kim, Da Hye Hong, Su Hyun Lee, Su Jeong Assefa, Freshet Kim, Gi-Young Park, Eui Kyun Park, Joung-Hyun Lee, Bae-Jin Jeon, You-Jin Choi, Yung Hyun Gamma Aminobutyric Acid-Enriched Fermented Oyster (Crassostrea gigas) Increases the Length of the Growth Plate on the Proximal Tibia Bone in Sprague-Dawley Rats |
title | Gamma Aminobutyric Acid-Enriched Fermented Oyster (Crassostrea gigas) Increases the Length of the Growth Plate on the Proximal Tibia Bone in Sprague-Dawley Rats |
title_full | Gamma Aminobutyric Acid-Enriched Fermented Oyster (Crassostrea gigas) Increases the Length of the Growth Plate on the Proximal Tibia Bone in Sprague-Dawley Rats |
title_fullStr | Gamma Aminobutyric Acid-Enriched Fermented Oyster (Crassostrea gigas) Increases the Length of the Growth Plate on the Proximal Tibia Bone in Sprague-Dawley Rats |
title_full_unstemmed | Gamma Aminobutyric Acid-Enriched Fermented Oyster (Crassostrea gigas) Increases the Length of the Growth Plate on the Proximal Tibia Bone in Sprague-Dawley Rats |
title_short | Gamma Aminobutyric Acid-Enriched Fermented Oyster (Crassostrea gigas) Increases the Length of the Growth Plate on the Proximal Tibia Bone in Sprague-Dawley Rats |
title_sort | gamma aminobutyric acid-enriched fermented oyster (crassostrea gigas) increases the length of the growth plate on the proximal tibia bone in sprague-dawley rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582314/ https://www.ncbi.nlm.nih.gov/pubmed/32977643 http://dx.doi.org/10.3390/molecules25194375 |
work_keys_str_mv | AT leehyesook gammaaminobutyricacidenrichedfermentedoystercrassostreagigasincreasesthelengthofthegrowthplateontheproximaltibiaboneinspraguedawleyrats AT hwangbohyun gammaaminobutyricacidenrichedfermentedoystercrassostreagigasincreasesthelengthofthegrowthplateontheproximaltibiaboneinspraguedawleyrats AT jiseonyeong gammaaminobutyricacidenrichedfermentedoystercrassostreagigasincreasesthelengthofthegrowthplateontheproximaltibiaboneinspraguedawleyrats AT kimminyeong gammaaminobutyricacidenrichedfermentedoystercrassostreagigasincreasesthelengthofthegrowthplateontheproximaltibiaboneinspraguedawleyrats AT kimsoyoung gammaaminobutyricacidenrichedfermentedoystercrassostreagigasincreasesthelengthofthegrowthplateontheproximaltibiaboneinspraguedawleyrats AT kimdahye gammaaminobutyricacidenrichedfermentedoystercrassostreagigasincreasesthelengthofthegrowthplateontheproximaltibiaboneinspraguedawleyrats AT hongsuhyun gammaaminobutyricacidenrichedfermentedoystercrassostreagigasincreasesthelengthofthegrowthplateontheproximaltibiaboneinspraguedawleyrats AT leesujeong gammaaminobutyricacidenrichedfermentedoystercrassostreagigasincreasesthelengthofthegrowthplateontheproximaltibiaboneinspraguedawleyrats AT assefafreshet gammaaminobutyricacidenrichedfermentedoystercrassostreagigasincreasesthelengthofthegrowthplateontheproximaltibiaboneinspraguedawleyrats AT kimgiyoung gammaaminobutyricacidenrichedfermentedoystercrassostreagigasincreasesthelengthofthegrowthplateontheproximaltibiaboneinspraguedawleyrats AT parkeuikyun gammaaminobutyricacidenrichedfermentedoystercrassostreagigasincreasesthelengthofthegrowthplateontheproximaltibiaboneinspraguedawleyrats AT parkjounghyun gammaaminobutyricacidenrichedfermentedoystercrassostreagigasincreasesthelengthofthegrowthplateontheproximaltibiaboneinspraguedawleyrats AT leebaejin gammaaminobutyricacidenrichedfermentedoystercrassostreagigasincreasesthelengthofthegrowthplateontheproximaltibiaboneinspraguedawleyrats AT jeonyoujin gammaaminobutyricacidenrichedfermentedoystercrassostreagigasincreasesthelengthofthegrowthplateontheproximaltibiaboneinspraguedawleyrats AT choiyunghyun gammaaminobutyricacidenrichedfermentedoystercrassostreagigasincreasesthelengthofthegrowthplateontheproximaltibiaboneinspraguedawleyrats |