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Site-specific epitope insertion into recombinant proteins using the MAP tag system

The MAP tag system comprises a 14-residue peptide derived from mouse podoplanin and its high-affinity monoclonal antibody PMab-1. We determined the crystal structure of PMab-1 complexed with the MAP tag peptide and found that the recognition required only the N-terminal 8 residues of MAP tag sequenc...

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Detalles Bibliográficos
Autores principales: Wakasa, Ayami, Kaneko, Mika K, Kato, Yukinari, Takagi, Junichi, Arimori, Takao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7585734/
https://www.ncbi.nlm.nih.gov/pubmed/32386302
http://dx.doi.org/10.1093/jb/mvaa054