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A scalable suspension insect cell transfection method for production of baculoviruses with low amplification passages
Baculovirus expression vector systems (BEVS) have been widely used for production of recombinant proteins in insect cells. However, baculoviruses superinfection and repeated passages originate defective interfering particle (DIP) mutants, which is a limitation to a continuous large-scale production....
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7586131/ https://www.ncbi.nlm.nih.gov/pubmed/33134099 http://dx.doi.org/10.1016/j.mex.2020.101103 |