Cargando…

A scalable suspension insect cell transfection method for production of baculoviruses with low amplification passages

Baculovirus expression vector systems (BEVS) have been widely used for production of recombinant proteins in insect cells. However, baculoviruses superinfection and repeated passages originate defective interfering particle (DIP) mutants, which is a limitation to a continuous large-scale production....

Descripción completa

Detalles Bibliográficos
Autores principales:	Cabral, Aline Diniz, Garcia, Felipe Baena, da Costa, Renata Torres, Vasconcelos, Ligia Marinho Pereira, Uehara, Mabel, Santos, Edmar Silva, Sperança, Márcia Aparecida
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2020
Materias:	Protocol Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7586131/ https://www.ncbi.nlm.nih.gov/pubmed/33134099 http://dx.doi.org/10.1016/j.mex.2020.101103

Ejemplares similares

Transfection of insect cell in suspension for efficient baculovirus generation
por: Roest, S., et al.
Publicado: (2016)

Dataset on recombinant expression of an ancient chitinase gene from different species of Leishmania parasites in bacteria and in Spodoptera frugiperda cells using baculovirus
por: Cabral, Aline Diniz, et al.
Publicado: (2020)

Baculovirus and insect cell gene expression: review of baculovirus biotechnology.
por: Patterson, R M, et al.
Publicado: (1995)

Baculoviruses: Sophisticated Pathogens of Insects
por: Clem, Rollie J., et al.
Publicado: (2013)

Covert Infection of Insects by Baculoviruses
por: Williams, Trevor, et al.
Publicado: (2017)

Transcriptome analyses of insect cells to facilitate baculovirus-insect expression
por: Yu, Kai, et al.
Publicado: (2016)

Genome Scale Transcriptomics of Baculovirus-Insect Interactions
por: Nguyen, Quan, et al.
Publicado: (2013)

A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (cop-GFP) in Baculovirus Expression System
por: Shokrollahi, Narjes, et al.
Publicado: (2016)

A non-destructive enzymatic method to extract DNA from arthropod specimens: Implications for morphological and molecular studies
por: Santos, Daubian, et al.
Publicado: (2018)

Baculovirus as versatile vectors for protein expression in insect and mammalian cells
por: Kost, Thomas A, et al.
Publicado: (2005)

Scalable Passaging of Adherent Human Pluripotent Stem Cells
por: Nie, Ying, et al.
Publicado: (2014)

Expression of hemagglutinin protein from the avian influenza virus H5N1 in a baculovirus/insect cell system significantly enhanced by suspension culture
por: Nwe, Nitar, et al.
Publicado: (2006)

Insect cell lines and baculoviruses as effective biocontrol agents of forest pests
por: Caputo, Guido F, et al.
Publicado: (2011)

Population genomics supports baculoviruses as vectors of horizontal transfer of insect transposons
por: Gilbert, Clément, et al.
Publicado: (2014)

Baculovirus expression systems for production of recombinant proteins in insect and mammalian cells
por: Beljelarskaya, S. N.
Publicado: (2011)

Baculovirus as a highly efficient expression vector in insect and mammalian cells
por: HU, Yu‐chen
Publicado: (2005)

Baculovirus transit through insect cell membranes: A mechanistic approach
por: Zhang, Youhong, et al.
Publicado: (2020)

Genetic engineering of baculovirus-insect cell system to improve protein production
por: Hong, Minqing, et al.
Publicado: (2022)

High Efficiency Lipid-Based siRNA Transfection of Adipocytes in Suspension
por: Kilroy, Gail, et al.
Publicado: (2009)

Suitable transfection methods for single particle tracing in plant suspension cells
por: Göhring, Janett, et al.
Publicado: (2014)

Display of a Maize cDNA library on baculovirus infected insect cells
por: Meller Harel, Helene Y, et al.
Publicado: (2008)

Developing Models of Disease Transmission: Insights from Ecological Studies of Insects and Their Baculoviruses
por: Elderd, Bret D.
Publicado: (2013)

Impact of Group II Baculovirus IAPs on Virus-Induced Apoptosis in Insect Cells
por: Zheng, Hao, et al.
Publicado: (2022)

Downregulation of lysosomal trafficking in ARPE19 cells leads to decreased transfection efficiency at high passage
por: Hood, Erika M.S., et al.
Publicado: (2023)

MultiBac: Baculovirus-Mediated Multigene DNA Cargo Delivery in Insect and Mammalian Cells
por: Gupta, Kapil, et al.
Publicado: (2019)

Production of CCHF virus-like particle by a baculovirus-insect cell expression system
por: Zhou, Zhao-rui, et al.
Publicado: (2011)

Expression of an antiviral protein from Lonomia obliqua hemolymph in baculovirus/insect cell system
por: Carmo, A.C.V., et al.
Publicado: (2012)

Baculovirus-free insect cell expression system for high yield antibody and antigen production
por: Korn, Janin, et al.
Publicado: (2020)

Rapid, scalable, and low-cost purification of recombinant adeno-associated virus produced by baculovirus expression vector system
por: Buclez, Pierre-Olivier, et al.
Publicado: (2016)

Production and purification of VP2 protein of porcine parvovirus expressed in an insect-baculovirus cell system
por: Zhou, Hongchao, et al.
Publicado: (2010)

Residual Baculovirus in Insect Cell-Derived Influenza Virus-Like Particle Preparations Enhances Immunogenicity
por: Margine, Irina, et al.
Publicado: (2012)

Optimization of canine interleukin-12 production using a baculovirus insect cell expression system
por: de Pinheiro, Cristiane Garboggini Melo, et al.
Publicado: (2016)

A new single-step protocol for rapid baculovirus-driven protein production in insect cells
por: Scholz, Judith, et al.
Publicado: (2017)

Expressing Double-Stranded RNAs of Insect Hormone-Related Genes Enhances Baculovirus Insecticidal Activity
por: Liu, Zheming, et al.
Publicado: (2019)

Baculovirus expression of cloned porcine arterivirus generates infectious particles in both insect and mammalian cells
por: Zheng, Hao, et al.
Publicado: (2010)

Accelerated induction of apoptosis in insect cells by baculovirus‐expressed SARS‐CoV membrane protein
por: Lai, Chia-Wei, et al.
Publicado: (2006)

The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics
por: Sari, Duygu, et al.
Publicado: (2015)

Scalable continuous-flow electroporation platform enabling T cell transfection for cellular therapy manufacturing
por: VanderBurgh, Jacob A., et al.
Publicado: (2023)

Rotavirus A-specific single-domain antibodies produced in baculovirus-infected insect larvae are protective in vivo
por: Gómez-Sebastián, Silvia, et al.
Publicado: (2012)

Two Novel 30K Proteins Overexpressed in Baculovirus System and Their Antiapoptotic Effect in Insect and Mammalian Cells
por: Yu, Wei, et al.
Publicado: (2013)

Cannot write session to /tmp/vufind_sessions/sess_mthme6e2kih8ji5n347u0b4irt