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Vitamin D is an endogenous partial agonist of the transient receptor potential vanilloid 1 channel

KEY POINTS: 25‐Hydroxyvitamin D (25OHD) is a partial agonist of TRPV1 whereby 25OHD can weakly activate TRPV1 yet antagonize the stimulatory effects of the full TRPV1 agonists capsaicin and oleoyl dopamine. 25OHD binds to TRPV1 within the same vanilloid binding pocket as capsaicin. 25OHD inhibits th...

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Autores principales: Long, Wentong, Fatehi, Mohammad, Soni, Shubham, Panigrahi, Rashmi, Philippaert, Koenraad, Yu, Yi, Kelly, Rees, Boonen, Brett, Barr, Amy, Golec, Dominic, Campbell, Scott A., Ondrusova, Katarina, Hubert, Matt, Baldwin, Troy, Lemieux, M. Joanne, Light, Peter E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7589233/
https://www.ncbi.nlm.nih.gov/pubmed/32721035
http://dx.doi.org/10.1113/JP279961
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author Long, Wentong
Fatehi, Mohammad
Soni, Shubham
Panigrahi, Rashmi
Philippaert, Koenraad
Yu, Yi
Kelly, Rees
Boonen, Brett
Barr, Amy
Golec, Dominic
Campbell, Scott A.
Ondrusova, Katarina
Hubert, Matt
Baldwin, Troy
Lemieux, M. Joanne
Light, Peter E.
author_facet Long, Wentong
Fatehi, Mohammad
Soni, Shubham
Panigrahi, Rashmi
Philippaert, Koenraad
Yu, Yi
Kelly, Rees
Boonen, Brett
Barr, Amy
Golec, Dominic
Campbell, Scott A.
Ondrusova, Katarina
Hubert, Matt
Baldwin, Troy
Lemieux, M. Joanne
Light, Peter E.
author_sort Long, Wentong
collection PubMed
description KEY POINTS: 25‐Hydroxyvitamin D (25OHD) is a partial agonist of TRPV1 whereby 25OHD can weakly activate TRPV1 yet antagonize the stimulatory effects of the full TRPV1 agonists capsaicin and oleoyl dopamine. 25OHD binds to TRPV1 within the same vanilloid binding pocket as capsaicin. 25OHD inhibits the potentiating effects of PKC‐mediated TRPV1 activity. 25OHD reduces T‐cell activation and trigeminal neuron calcium signalling mediated by TRPV1 activity. These results provide evidence that TRPV1 is a novel receptor for the biological actions of vitamin D in addition to the well‐documented effects of vitamin D upon the nuclear vitamin D receptor. The results may have important implications for our current understanding of certain diseases where TRPV1 and vitamin D deficiency have been implicated, such as chronic pain and autoimmune diseases, such as type 1 diabetes. ABSTRACT: The capsaicin receptor TRPV1 plays an important role in nociception, inflammation and immunity and its activity is regulated by exogenous and endogenous lipophilic ligands. As vitamin D is lipophilic and involved in similar biological processes as TRPV1, we hypothesized that it directly regulates TRPV1 activity and function. Our calcium imaging and electrophysiological data demonstrate that vitamin D (25‐hydroxyvitamin D (25OHD) and 1,25‐hydroxyvitamin D (1,25OHD)) can weakly activate TRPV1 at physiologically relevant concentrations (100 nM). Furthermore, both 25OHD and 1,25OHD can inhibit capsaicin‐induced TRPV1 activity (IC(50) = 34.3 ± 0.2 and 11.5 ± 0.9 nM, respectively), but not pH‐induced TRPV1 activity, suggesting that vitamin D interacts with TRPV1 in the same region as the TRPV1 agonist capsaicin. This hypothesis is supported by our in silico TRPV1 structural modelling studies, which place 25OHD in the same binding region as capsaicin. 25OHD also attenuates PKC‐dependent TRPV1 potentiation via interactions with a known PKC phospho‐acceptor residue in TRPV1. To provide evidence for a physiological role for the interaction of vitamin D with TRPV1, we employed two different cellular models known to express TRPV1: mouse CD4(+) T‐cells and trigeminal neurons. Our results indicate that 25OHD reduces TRPV1‐induced cytokine release from T‐cells and capsaicin‐induced calcium activity in trigeminal neurons. In summary, we provide evidence that vitamin D is a novel endogenous regulator of TRPV1 channel activity that may play an important physiological role in addition to its known effects through the canonical nuclear vitamin D receptor pathway.
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spelling pubmed-75892332020-10-30 Vitamin D is an endogenous partial agonist of the transient receptor potential vanilloid 1 channel Long, Wentong Fatehi, Mohammad Soni, Shubham Panigrahi, Rashmi Philippaert, Koenraad Yu, Yi Kelly, Rees Boonen, Brett Barr, Amy Golec, Dominic Campbell, Scott A. Ondrusova, Katarina Hubert, Matt Baldwin, Troy Lemieux, M. Joanne Light, Peter E. J Physiol Molecular and Cellular KEY POINTS: 25‐Hydroxyvitamin D (25OHD) is a partial agonist of TRPV1 whereby 25OHD can weakly activate TRPV1 yet antagonize the stimulatory effects of the full TRPV1 agonists capsaicin and oleoyl dopamine. 25OHD binds to TRPV1 within the same vanilloid binding pocket as capsaicin. 25OHD inhibits the potentiating effects of PKC‐mediated TRPV1 activity. 25OHD reduces T‐cell activation and trigeminal neuron calcium signalling mediated by TRPV1 activity. These results provide evidence that TRPV1 is a novel receptor for the biological actions of vitamin D in addition to the well‐documented effects of vitamin D upon the nuclear vitamin D receptor. The results may have important implications for our current understanding of certain diseases where TRPV1 and vitamin D deficiency have been implicated, such as chronic pain and autoimmune diseases, such as type 1 diabetes. ABSTRACT: The capsaicin receptor TRPV1 plays an important role in nociception, inflammation and immunity and its activity is regulated by exogenous and endogenous lipophilic ligands. As vitamin D is lipophilic and involved in similar biological processes as TRPV1, we hypothesized that it directly regulates TRPV1 activity and function. Our calcium imaging and electrophysiological data demonstrate that vitamin D (25‐hydroxyvitamin D (25OHD) and 1,25‐hydroxyvitamin D (1,25OHD)) can weakly activate TRPV1 at physiologically relevant concentrations (100 nM). Furthermore, both 25OHD and 1,25OHD can inhibit capsaicin‐induced TRPV1 activity (IC(50) = 34.3 ± 0.2 and 11.5 ± 0.9 nM, respectively), but not pH‐induced TRPV1 activity, suggesting that vitamin D interacts with TRPV1 in the same region as the TRPV1 agonist capsaicin. This hypothesis is supported by our in silico TRPV1 structural modelling studies, which place 25OHD in the same binding region as capsaicin. 25OHD also attenuates PKC‐dependent TRPV1 potentiation via interactions with a known PKC phospho‐acceptor residue in TRPV1. To provide evidence for a physiological role for the interaction of vitamin D with TRPV1, we employed two different cellular models known to express TRPV1: mouse CD4(+) T‐cells and trigeminal neurons. Our results indicate that 25OHD reduces TRPV1‐induced cytokine release from T‐cells and capsaicin‐induced calcium activity in trigeminal neurons. In summary, we provide evidence that vitamin D is a novel endogenous regulator of TRPV1 channel activity that may play an important physiological role in addition to its known effects through the canonical nuclear vitamin D receptor pathway. John Wiley and Sons Inc. 2020-08-13 2020-10-01 /pmc/articles/PMC7589233/ /pubmed/32721035 http://dx.doi.org/10.1113/JP279961 Text en © 2020 The Authors. The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Molecular and Cellular
Long, Wentong
Fatehi, Mohammad
Soni, Shubham
Panigrahi, Rashmi
Philippaert, Koenraad
Yu, Yi
Kelly, Rees
Boonen, Brett
Barr, Amy
Golec, Dominic
Campbell, Scott A.
Ondrusova, Katarina
Hubert, Matt
Baldwin, Troy
Lemieux, M. Joanne
Light, Peter E.
Vitamin D is an endogenous partial agonist of the transient receptor potential vanilloid 1 channel
title Vitamin D is an endogenous partial agonist of the transient receptor potential vanilloid 1 channel
title_full Vitamin D is an endogenous partial agonist of the transient receptor potential vanilloid 1 channel
title_fullStr Vitamin D is an endogenous partial agonist of the transient receptor potential vanilloid 1 channel
title_full_unstemmed Vitamin D is an endogenous partial agonist of the transient receptor potential vanilloid 1 channel
title_short Vitamin D is an endogenous partial agonist of the transient receptor potential vanilloid 1 channel
title_sort vitamin d is an endogenous partial agonist of the transient receptor potential vanilloid 1 channel
topic Molecular and Cellular
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7589233/
https://www.ncbi.nlm.nih.gov/pubmed/32721035
http://dx.doi.org/10.1113/JP279961
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