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Physiological characterization of a new thermotolerant yeast strain isolated during Brazilian ethanol production, and its application in high-temperature fermentation

BACKGROUND: The use of thermotolerant yeast strains can improve the efficiency of ethanol fermentation, allowing fermentation to occur at temperatures higher than 40 °C. This characteristic could benefit traditional bio-ethanol production and allow simultaneous saccharification and fermentation (SSF...

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Autores principales: Prado, Cleiton D., Mandrujano, Gustavo P. L., Souza, Jonas. P., Sgobbi, Flávia B., Novaes, Hosana R., da Silva, João P. M. O., Alves, Mateus H. R., Eliodório, Kevy P., Cunha, Gabriel C. G., Giudici, Reinaldo, Procópio, Diele P., Basso, Thiago O., Malavazi, Iran, Cunha, Anderson F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590731/
https://www.ncbi.nlm.nih.gov/pubmed/33117432
http://dx.doi.org/10.1186/s13068-020-01817-6
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author Prado, Cleiton D.
Mandrujano, Gustavo P. L.
Souza, Jonas. P.
Sgobbi, Flávia B.
Novaes, Hosana R.
da Silva, João P. M. O.
Alves, Mateus H. R.
Eliodório, Kevy P.
Cunha, Gabriel C. G.
Giudici, Reinaldo
Procópio, Diele P.
Basso, Thiago O.
Malavazi, Iran
Cunha, Anderson F.
author_facet Prado, Cleiton D.
Mandrujano, Gustavo P. L.
Souza, Jonas. P.
Sgobbi, Flávia B.
Novaes, Hosana R.
da Silva, João P. M. O.
Alves, Mateus H. R.
Eliodório, Kevy P.
Cunha, Gabriel C. G.
Giudici, Reinaldo
Procópio, Diele P.
Basso, Thiago O.
Malavazi, Iran
Cunha, Anderson F.
author_sort Prado, Cleiton D.
collection PubMed
description BACKGROUND: The use of thermotolerant yeast strains can improve the efficiency of ethanol fermentation, allowing fermentation to occur at temperatures higher than 40 °C. This characteristic could benefit traditional bio-ethanol production and allow simultaneous saccharification and fermentation (SSF) of starch or lignocellulosic biomass. RESULTS: We identified and characterized the physiology of a new thermotolerant strain (LBGA-01) able to ferment at 40 °C, which is more resistant to stressors as sucrose, furfural and ethanol than CAT-1 industrial strain. Furthermore, this strain showed similar CAT-1 resistance to acetic acid and lactic acid, and it was also able to change the pattern of genes involved in sucrose assimilation (SUC2 and AGT1). Genes related to the production of proteins involved in secondary products of fermentation were also differentially regulated at 40 °C, with reduced expression of genes involved in the formation of glycerol (GPD2), acetate (ALD6 and ALD4), and acetyl-coenzyme A synthetase 2 (ACS2). Fermentation tests using chemostats showed that LBGA-01 had an excellent performance in ethanol production in high temperature. CONCLUSION: The thermotolerant LBGA-01 strain modulates the production of key genes, changing metabolic pathways during high-temperature fermentation, and increasing its resistance to high concentration of ethanol, sugar, lactic acid, acetic acid, and furfural. Results indicate that this strain can be used to improve first- and second-generation ethanol production in Brazil.
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spelling pubmed-75907312020-10-27 Physiological characterization of a new thermotolerant yeast strain isolated during Brazilian ethanol production, and its application in high-temperature fermentation Prado, Cleiton D. Mandrujano, Gustavo P. L. Souza, Jonas. P. Sgobbi, Flávia B. Novaes, Hosana R. da Silva, João P. M. O. Alves, Mateus H. R. Eliodório, Kevy P. Cunha, Gabriel C. G. Giudici, Reinaldo Procópio, Diele P. Basso, Thiago O. Malavazi, Iran Cunha, Anderson F. Biotechnol Biofuels Research BACKGROUND: The use of thermotolerant yeast strains can improve the efficiency of ethanol fermentation, allowing fermentation to occur at temperatures higher than 40 °C. This characteristic could benefit traditional bio-ethanol production and allow simultaneous saccharification and fermentation (SSF) of starch or lignocellulosic biomass. RESULTS: We identified and characterized the physiology of a new thermotolerant strain (LBGA-01) able to ferment at 40 °C, which is more resistant to stressors as sucrose, furfural and ethanol than CAT-1 industrial strain. Furthermore, this strain showed similar CAT-1 resistance to acetic acid and lactic acid, and it was also able to change the pattern of genes involved in sucrose assimilation (SUC2 and AGT1). Genes related to the production of proteins involved in secondary products of fermentation were also differentially regulated at 40 °C, with reduced expression of genes involved in the formation of glycerol (GPD2), acetate (ALD6 and ALD4), and acetyl-coenzyme A synthetase 2 (ACS2). Fermentation tests using chemostats showed that LBGA-01 had an excellent performance in ethanol production in high temperature. CONCLUSION: The thermotolerant LBGA-01 strain modulates the production of key genes, changing metabolic pathways during high-temperature fermentation, and increasing its resistance to high concentration of ethanol, sugar, lactic acid, acetic acid, and furfural. Results indicate that this strain can be used to improve first- and second-generation ethanol production in Brazil. BioMed Central 2020-10-27 /pmc/articles/PMC7590731/ /pubmed/33117432 http://dx.doi.org/10.1186/s13068-020-01817-6 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Prado, Cleiton D.
Mandrujano, Gustavo P. L.
Souza, Jonas. P.
Sgobbi, Flávia B.
Novaes, Hosana R.
da Silva, João P. M. O.
Alves, Mateus H. R.
Eliodório, Kevy P.
Cunha, Gabriel C. G.
Giudici, Reinaldo
Procópio, Diele P.
Basso, Thiago O.
Malavazi, Iran
Cunha, Anderson F.
Physiological characterization of a new thermotolerant yeast strain isolated during Brazilian ethanol production, and its application in high-temperature fermentation
title Physiological characterization of a new thermotolerant yeast strain isolated during Brazilian ethanol production, and its application in high-temperature fermentation
title_full Physiological characterization of a new thermotolerant yeast strain isolated during Brazilian ethanol production, and its application in high-temperature fermentation
title_fullStr Physiological characterization of a new thermotolerant yeast strain isolated during Brazilian ethanol production, and its application in high-temperature fermentation
title_full_unstemmed Physiological characterization of a new thermotolerant yeast strain isolated during Brazilian ethanol production, and its application in high-temperature fermentation
title_short Physiological characterization of a new thermotolerant yeast strain isolated during Brazilian ethanol production, and its application in high-temperature fermentation
title_sort physiological characterization of a new thermotolerant yeast strain isolated during brazilian ethanol production, and its application in high-temperature fermentation
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590731/
https://www.ncbi.nlm.nih.gov/pubmed/33117432
http://dx.doi.org/10.1186/s13068-020-01817-6
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