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Mechanistic Insight on the Interaction between OPN and Integrin ανβ3 in Osteoarthritis

Osteoarthritis (OA) is a joint disease characterized by cartilage degeneration. Osteopontin (OPN) is involved in the initiation, repair, and maintenance of metabolic homeostasis in normal articular cartilage. This study investigated the role of OPN and its interaction with the integrin ανβ3 receptor...

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Detalles Bibliográficos
Autores principales: Yuan, Yuhao, Liu, Qing, Wu, Ziyi, Luo, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7593734/
https://www.ncbi.nlm.nih.gov/pubmed/33134374
http://dx.doi.org/10.1155/2020/2905634
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author Yuan, Yuhao
Liu, Qing
Wu, Ziyi
Luo, Wei
author_facet Yuan, Yuhao
Liu, Qing
Wu, Ziyi
Luo, Wei
author_sort Yuan, Yuhao
collection PubMed
description Osteoarthritis (OA) is a joint disease characterized by cartilage degeneration. Osteopontin (OPN) is involved in the initiation, repair, and maintenance of metabolic homeostasis in normal articular cartilage. This study investigated the role of OPN and its interaction with the integrin ανβ3 receptor in the expression of hyaluronic acid (HA) in OA chondrocytes. Overexpression of OPN significantly increased the expression of integrin ανβ3 and hyaluronic acid synthases (HAS) and synthesis of HA. Depleting OPN in OA chondrocytes showed the opposite trend for integrin alpha;νβ3, HAS, and HA. Nonspecifically and specifically blocking integrin receptor using GRGDSP and integrin ανβ3 antibody downregulated HAS and HA; both were inhibited to similar extents. The expression of HAS and HA was predominantly regulated by the interaction between OPN and integrin ανβ3. Taken together, we have delineated the importance of the OPN/integrin ανβ3/HAS/HA axis in OA and identified OPN as a promising candidate for molecular therapy for use in patients with OA.
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spelling pubmed-75937342020-10-30 Mechanistic Insight on the Interaction between OPN and Integrin ανβ3 in Osteoarthritis Yuan, Yuhao Liu, Qing Wu, Ziyi Luo, Wei Biomed Res Int Research Article Osteoarthritis (OA) is a joint disease characterized by cartilage degeneration. Osteopontin (OPN) is involved in the initiation, repair, and maintenance of metabolic homeostasis in normal articular cartilage. This study investigated the role of OPN and its interaction with the integrin ανβ3 receptor in the expression of hyaluronic acid (HA) in OA chondrocytes. Overexpression of OPN significantly increased the expression of integrin ανβ3 and hyaluronic acid synthases (HAS) and synthesis of HA. Depleting OPN in OA chondrocytes showed the opposite trend for integrin alpha;νβ3, HAS, and HA. Nonspecifically and specifically blocking integrin receptor using GRGDSP and integrin ανβ3 antibody downregulated HAS and HA; both were inhibited to similar extents. The expression of HAS and HA was predominantly regulated by the interaction between OPN and integrin ανβ3. Taken together, we have delineated the importance of the OPN/integrin ανβ3/HAS/HA axis in OA and identified OPN as a promising candidate for molecular therapy for use in patients with OA. Hindawi 2020-10-20 /pmc/articles/PMC7593734/ /pubmed/33134374 http://dx.doi.org/10.1155/2020/2905634 Text en Copyright © 2020 Yuhao Yuan et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yuan, Yuhao
Liu, Qing
Wu, Ziyi
Luo, Wei
Mechanistic Insight on the Interaction between OPN and Integrin ανβ3 in Osteoarthritis
title Mechanistic Insight on the Interaction between OPN and Integrin ανβ3 in Osteoarthritis
title_full Mechanistic Insight on the Interaction between OPN and Integrin ανβ3 in Osteoarthritis
title_fullStr Mechanistic Insight on the Interaction between OPN and Integrin ανβ3 in Osteoarthritis
title_full_unstemmed Mechanistic Insight on the Interaction between OPN and Integrin ανβ3 in Osteoarthritis
title_short Mechanistic Insight on the Interaction between OPN and Integrin ανβ3 in Osteoarthritis
title_sort mechanistic insight on the interaction between opn and integrin ανβ3 in osteoarthritis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7593734/
https://www.ncbi.nlm.nih.gov/pubmed/33134374
http://dx.doi.org/10.1155/2020/2905634
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