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Masking terminal neo-epitopes of linear peptides through glycosylation favours immune responses towards core epitopes producing parental protein bound antibodies

Glycosylation of hydrophobic peptides at one terminus effectively increases their water-solubility, and conjugation through the opposing end to a carrier protein, renders them more immunogenic. Moreover, the glycosylation minimizes antibody responses to potentially deleterious, non-productive termin...

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Autores principales: Pon, Robert, Marcil, Anne, Chen, Wangxue, Gadoury, Christine, Williams, Dean, Chan, Kenneth, Zhou, Hongyan, Ponce, Amalia, Paquet, Eric, Gurnani, Komal, Chattopadhyay, Anindita, Zou, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595224/
https://www.ncbi.nlm.nih.gov/pubmed/33116268
http://dx.doi.org/10.1038/s41598-020-75754-7
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author Pon, Robert
Marcil, Anne
Chen, Wangxue
Gadoury, Christine
Williams, Dean
Chan, Kenneth
Zhou, Hongyan
Ponce, Amalia
Paquet, Eric
Gurnani, Komal
Chattopadhyay, Anindita
Zou, Wei
author_facet Pon, Robert
Marcil, Anne
Chen, Wangxue
Gadoury, Christine
Williams, Dean
Chan, Kenneth
Zhou, Hongyan
Ponce, Amalia
Paquet, Eric
Gurnani, Komal
Chattopadhyay, Anindita
Zou, Wei
author_sort Pon, Robert
collection PubMed
description Glycosylation of hydrophobic peptides at one terminus effectively increases their water-solubility, and conjugation through the opposing end to a carrier protein, renders them more immunogenic. Moreover, the glycosylation minimizes antibody responses to potentially deleterious, non-productive terminal neo-epitope regions of the peptides, and consequently shifts peptide immunogenicity towards the core amino acid residues. As proof of concept, glycopeptide-protein conjugates related to influenza hemagglutinin (HA), neuraminidase (NA), and the dimerization loop region of human epidermal growth factor receptor 2 (Her2), demonstrated a favorable production of core peptide specific antibodies as determined by ELISA studies. Furthermore, glycosylated Her2 peptide conjugate antisera were also shown to recognize full length Her2 protein by ELISA and at the cell surface through flow cytometry analysis. In contrast, unmasked peptide conjugates generated significant antibody populations that were specific to the terminal neo-epitope of the peptide immunogen that are notably absent in parental proteins. Antibodies generated in this manner to peptides in the dimerization loop of Her2 are also functional as demonstrated by the growth inhibition of Her2 expressing SKBR3 carcinoma cells. This method provides a technique to tailor-make epitope-specific antibodies that may facilitate vaccine, therapeutic and diagnostic antibody development.
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spelling pubmed-75952242020-10-29 Masking terminal neo-epitopes of linear peptides through glycosylation favours immune responses towards core epitopes producing parental protein bound antibodies Pon, Robert Marcil, Anne Chen, Wangxue Gadoury, Christine Williams, Dean Chan, Kenneth Zhou, Hongyan Ponce, Amalia Paquet, Eric Gurnani, Komal Chattopadhyay, Anindita Zou, Wei Sci Rep Article Glycosylation of hydrophobic peptides at one terminus effectively increases their water-solubility, and conjugation through the opposing end to a carrier protein, renders them more immunogenic. Moreover, the glycosylation minimizes antibody responses to potentially deleterious, non-productive terminal neo-epitope regions of the peptides, and consequently shifts peptide immunogenicity towards the core amino acid residues. As proof of concept, glycopeptide-protein conjugates related to influenza hemagglutinin (HA), neuraminidase (NA), and the dimerization loop region of human epidermal growth factor receptor 2 (Her2), demonstrated a favorable production of core peptide specific antibodies as determined by ELISA studies. Furthermore, glycosylated Her2 peptide conjugate antisera were also shown to recognize full length Her2 protein by ELISA and at the cell surface through flow cytometry analysis. In contrast, unmasked peptide conjugates generated significant antibody populations that were specific to the terminal neo-epitope of the peptide immunogen that are notably absent in parental proteins. Antibodies generated in this manner to peptides in the dimerization loop of Her2 are also functional as demonstrated by the growth inhibition of Her2 expressing SKBR3 carcinoma cells. This method provides a technique to tailor-make epitope-specific antibodies that may facilitate vaccine, therapeutic and diagnostic antibody development. Nature Publishing Group UK 2020-10-28 /pmc/articles/PMC7595224/ /pubmed/33116268 http://dx.doi.org/10.1038/s41598-020-75754-7 Text en © Crown 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Pon, Robert
Marcil, Anne
Chen, Wangxue
Gadoury, Christine
Williams, Dean
Chan, Kenneth
Zhou, Hongyan
Ponce, Amalia
Paquet, Eric
Gurnani, Komal
Chattopadhyay, Anindita
Zou, Wei
Masking terminal neo-epitopes of linear peptides through glycosylation favours immune responses towards core epitopes producing parental protein bound antibodies
title Masking terminal neo-epitopes of linear peptides through glycosylation favours immune responses towards core epitopes producing parental protein bound antibodies
title_full Masking terminal neo-epitopes of linear peptides through glycosylation favours immune responses towards core epitopes producing parental protein bound antibodies
title_fullStr Masking terminal neo-epitopes of linear peptides through glycosylation favours immune responses towards core epitopes producing parental protein bound antibodies
title_full_unstemmed Masking terminal neo-epitopes of linear peptides through glycosylation favours immune responses towards core epitopes producing parental protein bound antibodies
title_short Masking terminal neo-epitopes of linear peptides through glycosylation favours immune responses towards core epitopes producing parental protein bound antibodies
title_sort masking terminal neo-epitopes of linear peptides through glycosylation favours immune responses towards core epitopes producing parental protein bound antibodies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595224/
https://www.ncbi.nlm.nih.gov/pubmed/33116268
http://dx.doi.org/10.1038/s41598-020-75754-7
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